Bershadskiĭ A D, Liustig T M
Tsitologiia. 1975 Jun;17(6):639-46.
On the surface of the nirtocellulose membrane filter (pore size 0.3--0.5 mem), normal mouse or hamster embryo fibroblasts formed discrete cell aggregates. Behaviour of transformed fibroblast-like cells of 9 different lines was compared with that of normal cells. Cells of 3 transformed lines grew on this substratum as a uniform monolayer displaying no tendency to aggregation. The following 3 cell lines exposed a slightly "patchy" cell distribution on the 3rd--4th day of cultivation but were unable to form discrete aggregates. The remaining 3 lines did form aggregates but the dynamics of aggregation and the final aggregation pattern for two of them were abnormal. Only one of the 9 investigated transformed lines had the normal aggregation behaviour. Hence, in the course of the neoplastic evolution, cells lose their ability fo form aggregates on the filter surface. Mechanisms of cell aggregation and possible reasons of differencies in the aggregation behaviour between normal and transformed cells, are discussed.
在硝化纤维素膜滤器（孔径0.3 - 0.5微米）表面，正常小鼠或仓鼠胚胎成纤维细胞形成离散的细胞聚集体。将9种不同系的转化成纤维样细胞的行为与正常细胞的行为进行了比较。3种转化系的细胞在该基质上生长形成均匀的单层，没有聚集的倾向。以下3种细胞系在培养的第3 - 4天呈现出稍微“斑驳”的细胞分布，但无法形成离散的聚集体。其余3种细胞系确实形成了聚集体，但其中两种的聚集动态和最终聚集模式是异常的。9个被研究的转化系中只有1个具有正常的聚集行为。因此，在肿瘤演变过程中，细胞失去了在滤器表面形成聚集体的能力。讨论了细胞聚集的机制以及正常细胞和转化细胞在聚集行为上差异的可能原因。
