Rosa S, Bignami M
Ann Ist Super Sanita. 1989;25(4):545-8.
We investigated growth control in mixed cultures of normal and oncogene-transformed mouse fibroblasts. NIH/3T3 transformed by v-myc, polyoma large T, polyoma middle T, v-ras and v-src showed comparable cloning efficiencies in agarized medium. However, when cultivated with an excess of normal cells (Balb/3T3, C3H10T1/2 or primary rat and hamster embryo cells) ras, src, and middle T-transformed cells were able to form "foci" of transformation on the layer of density arrested normal cells, whereas myc- and polyoma large T-transformed cells lacked this ability. Addition of the phorbol ester tumor promoter, phorbol-12,13-didecanoate, rescued proliferation and focus-formation by these nuclear oncogenes-transformed cell lines. Evidence is presented and discussed supporting a main role of intercellular communication between normal and transformed cells in modulating suppression or expression of the transformed phenotype.
我们研究了正常和癌基因转化的小鼠成纤维细胞混合培养物中的生长控制。经v-myc、多瘤大T抗原、多瘤中T抗原、v-ras和v-src转化的NIH/3T3细胞在琼脂培养基中显示出相当的克隆效率。然而，当与过量的正常细胞（Balb/3T3、C3H10T1/2或原代大鼠和仓鼠胚胎细胞）一起培养时，ras、src和中T抗原转化的细胞能够在密度停滞的正常细胞层上形成转化“灶”，而myc和多瘤大T抗原转化的细胞则缺乏这种能力。添加佛波酯肿瘤促进剂佛波醇-12,13-十二烷酸酯可挽救这些核癌基因转化细胞系的增殖和灶形成。本文提供并讨论了证据，支持正常细胞与转化细胞之间的细胞间通讯在调节转化表型的抑制或表达中起主要作用。
