Ibrahim N M, Romano J E, Troedsson M H, Crabo B G
Department of Clinical and Population Sciences, University of Minnesota, St Paul 55108, USA.
J Androl. 2001 Sep-Oct;22(5):863-77.
Scrotal contents of 2 rams were insulated for 96 hours and the fraction (as a percentage) of clusterin-positive cells (CPCs) and its relationship to semen quality was investigated. Semen collection was started 18 days before insulation and was terminated on day 78 and day 63 after insulation in animals 1 and 2, respectively. Sperm clusterin was localized by immunostaining with anti-bovine clusterin antibody (anti-bCAb) and fluorescein isothiocyanate-conjugated immunoglobulin G. Scrotal insulation led to deterioration of semen quality and increased the percentage of CPCs in both rams. Two types of sperm reactivity were observed: an extensive, intensive staining pattern (ESP); and a localized, less-intensive staining pattern (LSP). The percentage of ESP-CPCs began to increase from day 6 and reached 88.8% and 100% on day 15 after insulation in animals 1 and 2, respectively. The increase in CPCs coincided with the presence of a high percentage of teratoid forms (88.3%) in semen from animal 1, and detached heads (81.4%) in semen from animal 2. After normal semen production was restored on day 60 in animal 1, the percentages of ESP-CPCs and LSP-CPCs returned to preinsulation rates, whereas only the ESP-CPCs returned to normal in animal 2. A negative relationship was observed between ESP-CPCs and total sperm/ejaculate (r = -.62), motility (r = -.78), viability (r = -.68), and filtration rate (r = -.71) in semen from animal 1. Conversely, a positive relationship was seen between ESP-CPCs and total abnormal spermatozoa (r = .82). Similar results were obtained in semen from animal 2. CPCs were nearly completely absent in glass wool-Sephadex (GWS)-filtered semen, suggesting a role for clusterin in the process of trapping abnormal spermatozoa in the GWS filters. We conclude that aberrant spermatogenesis induced by scrotal insulation increases the percentage of CPCs in ram semen. We suggest that the percentage of CPCs in ram semen could be a useful marker in poor-quality ejaculates.
对2只公羊的阴囊内容物进行了96小时的隔热处理，并研究了聚集素阳性细胞（CPCs）的比例（以百分比表示）及其与精液质量的关系。在隔热处理前18天开始采集精液，在动物1和动物2中，分别在隔热处理后的第78天和第63天终止采集。用抗牛聚集素抗体（抗-bCAb）和异硫氰酸荧光素偶联的免疫球蛋白G进行免疫染色，对精子聚集素进行定位。阴囊隔热导致两只公羊的精液质量下降，CPCs的百分比增加。观察到两种类型的精子反应性：广泛、强烈的染色模式（ESP）；以及局部、较弱的染色模式（LSP）。在动物1和动物2中，ESP-CPCs的百分比分别从隔热处理后的第6天开始增加，在第15天分别达到88.8%和100%。CPCs的增加与动物1精液中高比例的类畸形形态（88.3%）以及动物2精液中分离的头部（81.4%）的存在相一致。在动物1中，正常精液产生在第60天恢复后，ESP-CPCs和LSP-CPCs的百分比恢复到隔热处理前的水平，而在动物2中只有ESP-CPCs恢复正常。在动物1的精液中，观察到ESP-CPCs与总精子数/射精量（r = -.62）、活力（r = -.78）、存活率（r = -.68）和过滤率（r = -.71）之间呈负相关。相反，在动物2的精液中，观察到ESP-CPCs与总异常精子数之间呈正相关（r = .82）。在玻璃棉-葡聚糖凝胶（GWS）过滤的精液中几乎完全没有CPCs，这表明聚集素在GWS过滤器中捕获异常精子的过程中发挥作用。我们得出结论，阴囊隔热诱导的异常精子发生增加了公羊精液中CPCs的百分比。我们建议，公羊精液中CPCs的百分比可能是劣质射精的一个有用指标。
