意大利儿科和年轻成人地中海贫血患者中GB病毒C/庚型肝炎病毒暴露情况

GB virus C/hepatitis G virus exposure in Italian pediatric and young adult thalassemic patients.

作者信息

Kondili L A, Chionne P, Dettori S, Badolato M C, Grosso A, Vania A, Rapicetta M

机构信息

Virology Laboratory, Istituto Superiore di Sanità, Rome, Italy.

出版信息

Infection. 2001 Aug;29(4):219-21. doi: 10.1007/s15010-001-9172-7.

DOI:10.1007/s15010-001-9172-7

PMID:11545484

Abstract

BACKGROUND

The aim was to estimate the prevalence and the persistence of GB virus C/hepatitis G virus (GBV-C/HGV) exposure markers in a group at high risk for transfusion-transmitted agents.

PATIENTS AND METHODS

Serum samples from 37 thalassemic patients were screened for GBV-C/HGV RNA by reverse transcription PCR (RT-PCR) and for antibodies to the envelope protein E2 of GBV-C/HGV (anti-E2).

RESULTS AND DISCUSSION

GBV-C/HGV RNA and anti-E2 were detected in 13 (35%) and 12 (32%) sera, respectively. Contemporary presence of both markers was found in one patient. GBV-C/HGV exposure was found in 24 patients (64.8%). Mean levels of liver enzymes were similar in both exposed and unexposed GBV-C/HGV groups. 33 out of 35 patients showed no change in GBV-C/HGV RNA and anti-E2 status in sera taken 6 months apart. The rate of persistent infection was 92.3% and the anti-E2 seroconversion rate was 23% for sera taken at least 6 months apart. The temporal overlap between anti-E2 seroconversion and loss of detectable GBV-C/HGV RNA may last more than 6 months.

摘要

背景

目的是评估一组输血传播病原体高危人群中GB病毒C/庚型肝炎病毒（GBV-C/HGV）暴露标志物的流行率和持续性。

患者与方法

采用逆转录聚合酶链反应（RT-PCR）对37例地中海贫血患者的血清样本进行GBV-C/HGV RNA筛查，并检测GBV-C/HGV包膜蛋白E2抗体（抗-E2）。

结果与讨论

分别在13份（35%）和12份（32%）血清中检测到GBV-C/HGV RNA和抗-E2。在1例患者中发现两种标志物同时存在。24例患者（64.8%）存在GBV-C/HGV暴露。GBV-C/HGV暴露组和未暴露组的肝酶平均水平相似。35例患者中有33例在间隔6个月采集的血清中GBV-C/HGV RNA和抗-E2状态无变化。对于间隔至少6个月采集的血清，持续感染率为92.3%，抗-E2血清转化率为23%。抗-E2血清转化与可检测到的GBV-C/HGV RNA消失之间的时间重叠可能持续超过6个月。