Rüdiger M, Köpke U, Prösch S, Rauprich P, Wauer R R, Herting E
Clinic of Neonatology, Charité Mitte, Humboldt University, Berlin, Germany.
Crit Care Med. 2001 Sep;29(9):1786-91. doi: 10.1097/00003246-200109000-00022.
Partial liquid ventilation with perfluorocarbons (PFC) might be used as a new ventilatory strategy to treat respiratory insufficiency in congenital pneumonia. The present study investigates for the first time effects of PFC on growth and viability of group B streptococci (GBS) and Escherichia coli, bacteria frequently causing congenital pneumonia.
Prospective, in vitro study.
Research laboratory in a university.
Group B streptococci 090 Ia HD Colindale and E. coli K12, JM 101.
E. coli (10(7)/mL) were grown in the absence or presence of different PFC (RM 101, PF 5080, FO 6167) for up to 6 hrs. To study bacterial viability, GBS (5 x 10(7)/mL) were incubated in saline with or without different PFC, PFC/surfactant emulsions, or surfactant (Curosurf) for up to 5 hrs. Every 2 hrs, the colony forming units were determined by plating different dilutions of bacteria on agar.
RM 101 or PF 5080 alone and in emulsions with surfactant had no effect on viability of GBS or growth of E. coli. For FO 6167, a previously described toxicity was found, even if 1 mL of GBS suspension was incubated with only 100 microL of FO 6167, verifying the experimental design that guarantees a PFC bacteria contact. The toxic effects were almost prevented by forming a PFC-in-surfactant emulsion but not by preincubation of GBS with surfactant and subsequent FO 6167 exposure.
RM 101 and PF 5080 did not influence bacterial growth in vitro; direct effects on bacterial proliferation during partial liquid ventilation in congenital pneumonia seem, therefore, unlikely. Interestingly, we found that the known toxic effects of FO 6167 can be prevented by covering PFC with a surfactant film. Surfactant reduced the cytotoxic effects of FO 6167, probably by preventing a direct contact between FO 6167 and the bacterial cell wall.
使用全氟化碳（PFC）进行部分液体通气可能成为治疗先天性肺炎呼吸功能不全的一种新的通气策略。本研究首次调查了PFC对B族链球菌（GBS）和大肠杆菌生长及生存能力的影响，这两种细菌是引起先天性肺炎的常见病原体。
前瞻性体外研究。
一所大学的研究实验室。
B族链球菌090 Ia HD 科林代尔株和大肠杆菌K12、JM 101。
大肠杆菌（10⁷/mL）在不存在或存在不同PFC（RM 101、PF 5080、FO 6167）的情况下培养长达6小时。为研究细菌生存能力，将GBS（5×10⁷/mL）在含有或不含有不同PFC、PFC/表面活性剂乳液或表面活性剂（固尔苏）的盐水中孵育长达5小时。每2小时，通过将不同稀释度的细菌接种在琼脂平板上测定菌落形成单位。
单独的RM 101或PF 5080以及与表面活性剂形成的乳液对GBS的生存能力或大肠杆菌的生长均无影响。对于FO 6167，即使仅将1 mL GBS悬液与100 μL FO 6167孵育，也发现了先前描述的毒性，这验证了保证PFC与细菌接触的实验设计。通过形成PFC在表面活性剂中的乳液几乎可以防止毒性作用，但GBS先用表面活性剂预孵育然后再接触FO 6167则不能防止毒性作用。
RM 101和PF 5080在体外不影响细菌生长；因此，它们在先天性肺炎部分液体通气期间对细菌增殖的直接影响似乎不太可能。有趣的是，我们发现用表面活性剂膜覆盖PFC可以防止FO 6167已知的毒性作用。表面活性剂降低了FO 6167的细胞毒性作用，可能是通过防止FO 6167与细菌细胞壁直接接触实现的。
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