Gregor Tobias, Schmalisch Gerd, Burkhardt Wolfram, Proquitté Hans, Wauer Roland R, Rüdiger Mario
Clinic for neonatology, Charité Campus Mitte, Schumannstrasse 21, 10098, Berlin, Germany.
Intensive Care Med. 2003 Aug;29(8):1354-60. doi: 10.1007/s00134-003-1733-z. Epub 2003 Apr 16.
Inhalation of perfluorocarbons (PFC) has been suggested as a new and promising technique of PFC delivery in animal models of severe lung injury. However, no in vitro data were available on the efficacy of PFC aerosolization during mechanical ventilation. Therefore, the aim of the in vitro study was to investigate the influence of physical properties of PFC and the ventilatory settings on the amount of PFC delivered into the lung.
In vitro lung model study.
University research laboratory.
Two different PFC (PF 5080 and PFOB) were aerosolized with a PariBoy jet nebulizer. Using a PFC selective adsorber, the effect of endotracheal tube size (2 mm and 3 mm diameter) on delivery of PFC was investigated. PFC delivery was estimated by continuous measurement of weight gain of the adsorber (adsorption rate). Finally, the influence of respiratory rate and tidal volume on adsorption rate (AR) was studied. AR was significantly reduced by a decreasing tube diameter and ranged from 1.45+/-0.03 ml/min (no tube) to 0.93+/-0.03 ml/min (2.0 mm) for PF 5080 (vapor pressure 51 mmHg) and from 0.49+/-0.06 ml/min to 0.32+/-0.04 ml/min for PFOB (11 mmHg). PFC-aerosolization into a ventilatory circuit with simulation of spontaneous tidal breathing (minute volume 600 ml) reduced AR to 0.16+/-0.02 ml/min. During mechanical ventilation, changes in respiratory rate and tidal volume, which reduce minute ventilation, caused a decrease in AR.
The amount of PFC that can be delivered into the lung by aerosolization is very small and is influenced by PFC properties, tube size, and ventilatory settings.
在严重肺损伤动物模型中，吸入全氟化碳（PFC）已被视为一种新的、有前景的PFC给药技术。然而，关于机械通气期间PFC雾化效果的体外数据尚不可得。因此，本体外研究的目的是探讨PFC的物理性质和通气设置对输送至肺内的PFC量的影响。
体外肺模型研究。
大学研究实验室。
使用PariBoy喷射雾化器雾化两种不同的PFC（PF 5080和全氟辛烷磺酸）。使用PFC选择性吸附器，研究气管导管尺寸（直径2 mm和3 mm）对PFC输送的影响。通过连续测量吸附器的重量增加（吸附率）来估计PFC的输送量。最后，研究呼吸频率和潮气量对吸附率（AR）的影响。对于PF 5080（蒸气压51 mmHg），随着导管直径减小，AR显著降低，范围从无导管时的1.45±0.03 ml/分钟降至2.0 mm导管时的0.93±0.03 ml/分钟；对于全氟辛烷磺酸（11 mmHg），AR范围从0.49±0.06 ml/分钟降至0.32±0.04 ml/分钟。在模拟自主潮式呼吸（分钟通气量600 ml）的通气回路中进行PFC雾化，可使AR降至0.16±0.02 ml/分钟。在机械通气期间，降低分钟通气量的呼吸频率和潮气量变化会导致AR降低。
通过雾化可输送至肺内的PFC量非常小，且受PFC性质、导管尺寸和通气设置的影响。
