Hamana K, Nakagawa Y
School of Health Sciences, Faculty of Medicine, Gunma University, Maebashi, Japan.
Microbios. 2001;106 Suppl 2:105-16.
Cellular polyamines of 58 strains belonging to the Flavobacterium-Flexibacter-Cytophaga-Sphingobacterium complex were analysed by HPLC. Homospermidine was found in all species of Flavobacterium, Chryseobacterium, Empedobacter, Myroides, Cellulophaga, Salegentibacter, Psychroserpens and Gelidibacter of the family Flavobacteriaceae. Flavobacterium ferrugineum located outside of this family also contained homospermidine. Cytophaga fermentans and C. xylanolytica belonging to the family Bacteroidaceae contained spermidine. Cytophaga marinoflava and C. latercula belonging to Flavobacteriaceae contained homospermidine. The Cytophaga hutchinsonii/C. aurantiaca group contained homospermidine which was the major polyamine in Flexibacter maritimus/ F. ovolyticus of the family Flavobacteriaceae. The Flexibacter sancti/F filiformis/ Cytophaga arvensicola group, F. elegans, F. ruber, F. canadensis, F. flexilis and F. tractuosus, were located separately in different six clusters, and contained homospermidine. The Flexibacter litoralis/F. polymorphus/F. aggregans group contained spermidine, which was detected in Flexibacter roseolus belonging to a divergent cluster. Sphingobacterium and Pedobacter species of the family Sphingobacteriaceae contained homospermidine. Polyamine profiles serve, as a phenotypic chemotaxonomic marker, for the classification of this complex.
采用高效液相色谱法对属于黄杆菌-屈挠杆菌-噬纤维菌-鞘氨醇杆菌复合体的58个菌株的细胞多胺进行了分析。在黄杆菌科的所有黄杆菌属、金黄杆菌属、土栖杆菌属、海杆菌属、纤维弧菌属、盐单胞菌属、嗜冷蛇菌属和嗜冷杆菌属物种中均发现了高亚精胺。不属于该科的铁锈色黄杆菌也含有高亚精胺。属于拟杆菌科的发酵噬纤维菌和木聚糖分解噬纤维菌含有亚精胺。属于黄杆菌科的海生噬纤维菌和侧生噬纤维菌含有高亚精胺。哈钦森噬纤维菌/橙色噬纤维菌组含有高亚精胺，高亚精胺是黄杆菌科的海生屈挠杆菌/溶卵屈挠杆菌中的主要多胺。圣洁屈挠杆菌/丝状屈挠杆菌/田野噬纤维菌组、优美屈挠杆菌、红色屈挠杆菌、加拿大屈挠杆菌、柔韧屈挠杆菌和蜿蜒屈挠杆菌分别位于不同的六个簇中，且含有高亚精胺。滨海屈挠杆菌/多形屈挠杆菌/聚集屈挠杆菌组含有亚精胺，在属于一个不同簇的玫瑰色屈挠杆菌中也检测到了亚精胺。鞘氨醇杆菌科的鞘氨醇杆菌属和 Pedobacter 属物种含有高亚精胺。多胺谱作为一种表型化学分类标记，可用于该复合体的分类。
