通过体内¹³C核磁共振光谱研究氧气对专性甲烷氧化细菌甲醇氧化的影响。

The effect of oxygen on methanol oxidation by an obligate methanotrophic bacterium studied by in vivo 13C nuclear magnetic resonance spectroscopy.

作者信息

Costa C, Vecherskaya M, Dijkema C, Stams A J

机构信息

Chemical Engineering Department, University of Salamanca, Spain.

出版信息

J Ind Microbiol Biotechnol. 2001 Jan-Feb;26(1-2):9-14.

PMID:11548754

Abstract

13C NMR was used to study the effect of oxygen on methanol oxidation by a type II methanotrophic bacterium isolated from a bioreactor in which methane was used as electron donor for denitrification. Under high (35-25%) oxygen conditions the first step of methanol oxidation to formaldehyde was much faster than the following conversions to formate and carbon dioxide. Due to this the accumulation of formaldehyde led to a poisoning of the cells. A more balanced conversion of 13C-labelled methanol to carbon dioxide was observed at low (1-5%) oxygen concentrations. In this case, formaldehyde was slowly converted to formate and carbon dioxide. Formaldehyde did not accumulate to inhibitory levels. The oxygen-dependent formation of formaldehyde and formate from methanol is discussed kinetically and thermodynamically.

摘要

利用13C核磁共振技术研究了氧气对从一个以甲烷作为反硝化电子供体的生物反应器中分离出的II型甲烷营养细菌氧化甲醇的影响。在高氧（35%-25%）条件下，甲醇氧化为甲醛的第一步比随后转化为甲酸和二氧化碳的过程快得多。因此，甲醛的积累导致细胞中毒。在低氧（1%-5%）浓度下，观察到13C标记的甲醇向二氧化碳的转化更为平衡。在这种情况下，甲醛缓慢转化为甲酸和二氧化碳。甲醛不会积累到抑制水平。从动力学和热力学角度讨论了甲醇中甲醛和甲酸的氧依赖性形成。