Haapaniemi J, Schrey A, Laurikainen E
Department of Otorhinolaryngology, Turku University Central Hospital, Finland.
Eur Arch Otorhinolaryngol. 2001 Jul;258(5):209-12. doi: 10.1007/s004050100341.
Use of laser Doppler flowmetry (LDF) to assess cochlear blood flow (CBF) in experimental animals has elucidated the complex mechanisms in CBF regulation. Some attempts have been made to measure CBF in humans, although earlier studies produced confusing data about the physical possibility of LDF penetrating the human tympanic cavity promontory. In this study, we used LDF in an attempt to observe capillary blood flow in vivo through the intact human promontory in order to develop a method for measuring CBF in human patients. Temporal bones from six human cadavers were removed and the promontory overlying the basal turn of the cochlea was drilled off and fixed in 10% formalin. Perpendicular bone thickness was measured at the site of the stria vascularis. Seven young rats were anesthetized and the carotid artery was cannulated for blood pressure recording. Capillary flow was registered with LDF from two sites, the kidney and the plantar area of a hind limb, using a custom-designed probe with 632.8 nm and 780 nm wavelengths. Intravenous epinephrine was used to evoke blood flow changes. The same measurements were repeated through the promontorial bone. The light attenuation induced by the promontorial bone was 79+/-28% for the 632.8 nm wavelength, and 22+/-15 to 65+/-13% for the 780 nm wavelength, depending on the thickness of the bone. The sensitivity in detecting minute changes was 78% and 91-96%, respectively, for the 632.8 nm and 780 nm wavelengths. This study indicates that it is possible to detect minute changes in capillary blood flow through the intact human promontory at the site of the stria vascularis.
使用激光多普勒血流仪（LDF）评估实验动物的耳蜗血流量（CBF），已经阐明了CBF调节的复杂机制。虽然早期研究关于LDF穿透人鼓室岬的物理可能性产生了令人困惑的数据，但人们已经进行了一些测量人体CBF的尝试。在本研究中，我们使用LDF试图通过完整的人岬在体内观察毛细血管血流，以便开发一种测量人类患者CBF的方法。从六具人类尸体上取下颞骨，钻掉覆盖耳蜗底转的岬，并固定在10%的福尔马林中。在血管纹部位测量垂直骨厚度。七只年轻大鼠麻醉后，将颈动脉插管用于记录血压。使用定制设计的波长为632.8 nm和780 nm的探头，通过LDF从两个部位记录毛细血管血流，即肾脏和后肢足底区域。静脉注射肾上腺素以引起血流变化。通过岬骨重复相同的测量。对于632.8 nm波长，岬骨引起的光衰减为79±28%，对于780 nm波长，根据骨厚度，光衰减为22±15%至65±13%。对于632.8 nm和780 nm波长，检测微小变化的灵敏度分别为78%和91 - 96%。本研究表明，在血管纹部位通过完整的人岬检测毛细血管血流的微小变化是可能的。
