Wang W, Gu Y, Miyamoto M, Hori H, Nagata N, Balamurugan A N, Touma M, Sakurai T, Inoue K
Department of Organ Reconstruction, Institute for Frontier Medical Sciences, Kyoto University, Japan.
Cell Transplant. 2001;10(4-5):465-71.
Microencapsulation of pancreatic islets represents a potentially effective method to prevent graft rejection in allotransplantation or xenotransplantation without the need of immunosuppression. Adequate insulin secretion and glucose responsiveness of microencapsulated pancreatic islets has been regarded as a prerequisite for successful transplantation. The microencapsulated pancreatic islets were respectively cultured in bFGF+ RPMI-1640 medium (bFGF+) or bFGF- RPMI-1640 medium (bFGF-) for 21 days. The functional activities of microencapsulated pancreatic islets were assessed by measuring basal insulin secretion and stimulated insulin release at different time points. The results revealed that microencapsulated pancreatic islets in the presence of bFGF demonstrated an increase in basal insulin secretion. Furthermore, microencapsulated pancreatic islets in the presence of bFGF demonstrated a marked stimulated insulin release and relative stability of stimulation indices (SI). The results in the perifusion study showed that microencapsulated pancreatic islets in the presence of bFGF maintained good glucose responsiveness over the course of culture period as well. These results indicate that bFGF has a beneficial effect on insulin secretion from microencapsulated pancreatic islets during in vitro culture. New strategies for preserving and improving function of microencapsulated pancreatic islets prior to transplantation may be developed by application of growth factors or other factors.
胰岛微囊化是一种潜在的有效方法，可在同种异体移植或异种移植中防止移植物排斥，而无需免疫抑制。微囊化胰岛充分的胰岛素分泌和葡萄糖反应性被视为成功移植的先决条件。将微囊化胰岛分别在碱性成纤维细胞生长因子（bFGF）+ RPMI - 1640培养基（bFGF +）或不含bFGF的RPMI - 1640培养基（bFGF -）中培养21天。通过在不同时间点测量基础胰岛素分泌和刺激后的胰岛素释放来评估微囊化胰岛的功能活性。结果显示，存在bFGF时微囊化胰岛的基础胰岛素分泌增加。此外，存在bFGF时微囊化胰岛表现出显著的刺激后胰岛素释放以及刺激指数（SI）的相对稳定性。灌流研究结果表明，存在bFGF时微囊化胰岛在培养期间也保持了良好的葡萄糖反应性。这些结果表明，bFGF在体外培养期间对微囊化胰岛的胰岛素分泌具有有益作用。通过应用生长因子或其他因素，可能会开发出在移植前保存和改善微囊化胰岛功能的新策略。
