Sato Hirohide, Kobayasi Taizou, Murakami Makoto, Kimura Toshihisa, Yamaguchi Akio, Nakagawara Gizou, Iwata Hiroo
Department of Surgery, Fukui Medical University, Fukui, Japan.
Pancreas. 2002 Oct;25(3):e42-9. doi: 10.1097/00006676-200210000-00020.
Porcine pancreatic islets have been difficult to preserve because isolated porcine islets tend to disaggregate to single cells and lose function under culture conditions. In the current study, the influence of agarose microencapsulation on the maintenance of the number and function of islets in culture preservations and the effect of culture preconditioning of microencapsulated porcine islets on xenogenic transplantation were investigated.
Porcine islets were isolated and then microencapsulated in 5% agarose membrane. The percentage of naked and microencapsulated islets remaining in the culture preservations was assessed. The effect of microencapsulation and culture on secretory function was investigated in vitro. The survival of overnight-cultured and 7-days-cultured microencapsulated islets in xenogenic transplantations was examined.
A good percentage of microencapsulated islets remained in the culture preservations. They could maintain good secretory functions in vitro after 7 days of culture. In addition, we observed a significant prolongation of mean islet survival by culture preconditioning.
The present findings suggest that microencapsulation is one of the useful preserving methods for maintenance of the number and function of cultured isolated porcine islets. Moreover, culture preconditioning is effective for improving islet survival and might be a good option leading to clinical success.
猪胰岛一直难以保存，因为分离出的猪胰岛在培养条件下往往会分散成单个细胞并丧失功能。在本研究中，我们探究了琼脂糖微囊化对培养保存中胰岛数量和功能维持的影响，以及微囊化猪胰岛的培养预处理对异种移植的效果。
分离猪胰岛，然后将其微囊化于5%琼脂糖膜中。评估培养保存中裸胰岛和微囊化胰岛的留存百分比。在体外研究微囊化和培养对分泌功能的影响。检测过夜培养和7天培养的微囊化胰岛在异种移植中的存活率。
相当比例的微囊化胰岛留存于培养保存中。培养7天后，它们在体外仍能保持良好的分泌功能。此外，我们观察到通过培养预处理，胰岛平均存活时间显著延长。
目前的研究结果表明，微囊化是维持培养的分离猪胰岛数量和功能的有效保存方法之一。此外，培养预处理对于提高胰岛存活率有效，可能是通向临床成功的一个良好选择。
