Iwata H, Amemiya H, Matsuda T, Takano H, Hayashi R, Akutsu T
National Cardiovascular Center Research Institute, Osaka, Japan.
Diabetes. 1989 Jan;38 Suppl 1:224-5. doi: 10.2337/diab.38.1.s224.
Islets were microencapsulated in agarose gel for examination of the possible use of microencapsulated islets as a bioartificial pancreas. Microencapsulated islets secreted insulin into the culture medium (RPMI-1640) and could rapidly increase their insulin release in response to a glucose challenge even after greater than 100 days. Hamster islets in groups of 400-1000 encapsulated in microbeads containing 11-14% (wt/wt) agarose were xenogenically transplanted into the peritoneal cavity of five diabetic mice. The longest normoglycemic period in these mice was 53 days, which was markedly longer than the normoglycemic period obtained by nonencapsulated islets. Agarose seems to be a suitable basic material for encapsulating islets, because the islets can easily be microencapsulated without any adverse effect on the islet function.
将胰岛微囊化于琼脂糖凝胶中，以研究微囊化胰岛作为生物人工胰腺的潜在用途。微囊化胰岛可向培养基（RPMI - 1640）中分泌胰岛素，即使在超过100天后，对葡萄糖刺激仍能迅速增加胰岛素释放。将400 - 1000个仓鼠胰岛封装于含11 - 14%（重量/重量）琼脂糖的微珠中，异种移植到5只糖尿病小鼠的腹腔内。这些小鼠最长的血糖正常期为53天，明显长于未封装胰岛所获得的血糖正常期。琼脂糖似乎是封装胰岛的合适基础材料，因为胰岛能够轻松地被微囊化，且对胰岛功能无任何不利影响。
