Wales Andrew D, Pearson Geoffrey R, Skuse Andrew M, Roe John M, Hayes Christine M, Cookson Adrian L, Woodward Martin J
Department of Pathology and Microbiology and *Department of Clinical Veterinary Science, University of Bristol Veterinary School, Langford, Bristol BS40 7DU and †Department of Bacterial Diseases, Veterinary Laboratories Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB.
J Med Microbiol. 2001 Sep;50(9):752-758. doi: 10.1099/0022-1317-50-9-752.
Four 6-day-old conventionally reared lambs were inoculated orally with a total of 10(9) cfu comprising equal numbers of four enterohaemorrhagic Escherichia coli (EHEC) O157:H7 strains. All animals remained clinically normal. Tissues were sampled under terminal anaesthesia at 12, 36, 60 and 84 h post inoculation (hpi). EHEC O157:H7 was cultured from most gastrointestinal tract sites. Small, sparse attaching and effacing (AE) lesions were found in the caecum at 12 and 36 hpi and in the terminal colon and rectum at 84 hpi. Organisms in the lesions were labelled specifically by an O157 antiserum. The results indicate that the well-characterised mechanisms for intimate attachment encoded by the locus for enterocyte effacement (LEE) of EHEC O157:H7 may contribute to the initial events, at least, of colonisation of sheep.
四只6日龄常规饲养的羔羊经口接种了总共10(9) 个菌落形成单位（cfu），其中包含数量相等的四种肠出血性大肠杆菌（EHEC）O157:H7菌株。所有动物临床状态均保持正常。在接种后12、36、60和84小时（hpi）进行终末麻醉时采集组织样本。在大多数胃肠道部位培养出了EHEC O157:H7。在接种后12和36小时，盲肠出现了小而稀疏的紧密黏附和脱落（AE）损伤，在接种后84小时，终末结肠和直肠出现了此类损伤。损伤部位的细菌被O157抗血清特异性标记。结果表明，EHEC O157:H7肠细胞脱落位点（LEE）编码的特征明确的紧密黏附机制可能至少在绵羊定植的初始事件中发挥作用。
