Shul'ga M A, Kats L N, Polkovnikova L N, Prozorovskiĭ S V
Zh Mikrobiol Epidemiol Immunobiol. 1975 Jun(6):78-81.
A study was made of the ultrastructure of stable L-forms of Nag vibrios aged 24 hours. Cells of all types of the L-forms had cytoplasmic membranes, and a three-layered structure, which was found not everywhere. Externally of the cytoplasmic membrane, in some areas of the individual cells there were revealed a plastic layer of cell wall and a basal membrane. However, in difference to bacterial forms of the vibryos, rigidity of the cell wall was disturbed, and the links between the cell wall and the cytoplasmic membrane were indetectable. There were regularly revealed lamellar of myelin-like membranous structures in the cytoplasm, which did not occur in bacterial forms, and also lamellar mesosomes. The latter were found in the sites of cell division. Viability of small bodies as the minimal reproductive forms of the L-cultures is confirmed by the presence in them of a nucleoid and of the binary division.
对培养24小时的稳定型那氏弧菌L型的超微结构进行了研究。所有类型L型的细胞都有细胞质膜,还有一种三层结构,但并非在所有地方都能找到。在细胞质膜外部,个别细胞的某些区域发现了一层可塑性的细胞壁层和一层基膜。然而,与弧菌的细菌形态不同,细胞壁的刚性受到干扰,且细胞壁与细胞质膜之间的连接无法检测到。在细胞质中经常发现有髓鞘样膜结构的片层,这在细菌形态中不存在,还发现了片层中体。后者在细胞分裂部位被发现。作为L型培养物最小繁殖形式的小体的活力通过其中存在拟核和二分裂得到证实。