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[L型菌的超微结构。II. 单核细胞增生李斯特菌的L型菌]

[Ultrastructure of L-forms. II. L-forms of Listeria monocytogenes].

作者信息

Konstantinova N D, Kats L N, Kotliarova G A, Fedotova I I

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1975 Feb(2):58-61.

PMID:164746
Abstract

A study was made of ultrathin sections of the stable l-forms of listeria obtained under the action of penicillin in meat-peptone-liver broth. A marked cellular polymorphism was found in the L-form culture: within the same colony cells differed in size, shape and fine structure. It is supposed that polymorphism could be partially explained by a different plasticity and premeability of cytoplasmic membrane in different types of cells of the same L-colony. The three-layer structure of the membrane does not always display the same distinctness in various L-colony cells and also in different areas of the cell surface. Structureless material of low electron density, possibly a defective murein or its precursor, was revealed on the membrane surface. Electrondense inclusion bodies, mesosomes of ring-shaped or more complicated structure and two-contour vesicles were found in the cytoplasm. The cells multiplied by budding, by binary and anomalies division participation of mesosomes in this process was not proved by the L-forms.

摘要

对在肉蛋白胨肝肉汤中青霉素作用下获得的李斯特菌稳定L型的超薄切片进行了研究。在L型培养物中发现了明显的细胞多态性:在同一菌落内,细胞在大小、形状和精细结构上存在差异。据推测,多态性可能部分是由于同一L菌落不同类型细胞中细胞质膜的可塑性和通透性不同所致。膜的三层结构在不同的L菌落细胞以及细胞表面的不同区域并不总是表现出相同的清晰度。在膜表面发现了低电子密度的无结构物质,可能是有缺陷的胞壁质或其前体。在细胞质中发现了电子致密的包涵体、环形或更复杂结构的中介体以及双轮廓小泡。细胞通过出芽、二分裂和异常分裂进行增殖,L型并未证明中介体参与了这一过程。

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