锰离子在多核苷酸磷酸化酶与2'-O-甲基化底物反应中的作用
Role of Mn2+ in the reaction of polynucleotide phosphorylase with 2'-O-methylated substrates.
作者信息
Siedlecki J A, Zmudzka B
出版信息
Acta Biochim Pol. 1975;22(2):163-8.
PMID:1154944
Abstract
Polymerization of 2'-O-methylcytidine-5'-diphosphate (CmDP) with polynucleotide phosphorylase in the presence of Mn2+ proceeds with 65% yield after 72 h, and in the presence of Mg2+ the yield does not exceed 10%. Phosphorolysis of poly 2'-O-methylcytidylic acid and poly 2'-O-methyluridylic acid, as well as exchange of the beta-phosphate group of CmDP in the presence of Mn2+ and Mg2+, proceed with a yield of only a few percent. A possible mechanism of Mn2+ action on CmDP polymerization is discussed.
摘要
在存在Mn2+的情况下，2'-O-甲基胞苷-5'-二磷酸（CmDP）与多核苷酸磷酸化酶聚合，72小时后产率为65%，而在存在Mg2+的情况下，产率不超过10%。聚2'-O-甲基胞苷酸和聚2'-O-甲基尿苷酸的磷酸解，以及在存在Mn2+和Mg2+的情况下CmDP的β-磷酸基团交换，产率仅为百分之几。讨论了Mn2+对CmDP聚合作用的可能机制。
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