聚-2'-脱氧-2'-氟胞苷酸：酶促合成、光谱表征及其与聚肌苷酸的相互作用。
Poly-2'-deoxy-2'-fluoro-cytidylic acid: enzymatic synthesis, spectroscopic characterization and interaction with poly-inosinic acid.
作者信息
Guschlbauer W, Blandin M, Drocourt J L, Thang M N
出版信息
Nucleic Acids Res. 1977 Jun;4(6):1933-43. doi: 10.1093/nar/4.6.1933.
Abstract
The polymerization of 2'deoxy-2'-fluoro-cytidine-diphosphate (dCflDP) by polynucleotide phosphorylase is barely detectable in the presence of Mg++ under usual experimental conditions for polymerization of nucleoside diphosphates. High concentrations of enzyme have to be used to accomplish the synthesis. Mn++ is a better activator than Mg++ for the reaction. cCflDP inhibits the polymerization of CDP and has a Km=8.8X10-3M, six times higher than CDP.- The polymer, poly (dCfl), ressembles in many respects poly(C), but not poly(dC): the acid selfstructure forms at similar pK's; interaction with poly(I) yields a 1:1 complex the CD spectrum of which is similar to that of poly(I).poly(C). Finally, the Tm's of poly(I).poly(dCfl) are comparable to those of poly(I).poly(C).
摘要
在核苷二磷酸聚合的常规实验条件下，在Mg++存在时，多核苷酸磷酸化酶催化2'-脱氧-2'-氟胞苷二磷酸（dCflDP）的聚合反应几乎检测不到。必须使用高浓度的酶才能完成合成。对于该反应，Mn++比Mg++是更好的激活剂。cCflDP抑制CDP的聚合反应，其Km = 8.8×10-3M，比CDP高六倍。聚合物聚（dCfl）在许多方面类似于聚（C），但不像聚（dC）：在相似的pK值下形成酸自结构；与聚（I）相互作用产生1:1复合物，其圆二色光谱与聚（I）·聚（C）的相似。最后，聚（I）·聚（dCfl）的熔解温度与聚（I）·聚（C）的相当。
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