前列腺按摩后尿液中前列腺癌的DNA检测

DNA-based detection of prostate cancer in urine after prostatic massage.

作者信息

Goessl C, Müller M, Heicappell R, Krause H, Straub B, Schrader M, Miller K

机构信息

Department of Urology, Benjamin Franklin Medical School, Free University of Berlin, Berlin, Germany.

出版信息

Urology. 2001 Sep;58(3):335-8. doi: 10.1016/s0090-4295(01)01268-7.

DOI:10.1016/s0090-4295(01)01268-7

PMID:11549474

Abstract

OBJECTIVES

Promoter hypermethylation of the glutathione-S-transferase P1 (GSTP1) gene is a specific feature of prostate cancer. This epigenetic DNA alteration served as the target for molecular detection of prostate cancer cells in urine sediments after prostatic massage.

METHODS

Bisulfite treatment followed by methylation-specific polymerase chain reaction was used to detect GSTP1 promoter hypermethylation in DNA isolated from urine sediments obtained after prostatic massage of men with and without prostate cancer.

RESULTS

GSTP1 promoter hypermethylation was demonstrated in the sediments of 1 (2%) of 45 patients diagnosed with benign prostatic hyperplasia, 2 (29%) of 7 patients with prostatic intraepithelial neoplasia, 15 (68%) of 22 patients with early, intracapsular cancer, and 14 (78%) of 18 patients with locally advanced or systemic prostate cancer, resulting in a specificity of 98% and an overall sensitivity of 73% for the detection of prostate cancer.

CONCLUSIONS

Urinalysis for GSTP1 promoter hypermethylation constitutes a sensitive and highly specific DNA-based marker for molecular detection of prostate cancer, including early stages.

摘要

目的

谷胱甘肽 - S - 转移酶P1（GSTP1）基因启动子高甲基化是前列腺癌的一个特异性特征。这种表观遗传的DNA改变可作为前列腺按摩后尿沉渣中前列腺癌细胞分子检测的靶点。

方法

采用亚硫酸氢盐处理后进行甲基化特异性聚合酶链反应，以检测前列腺癌患者和非前列腺癌患者前列腺按摩后尿沉渣中分离出的DNA中GSTP1启动子的高甲基化情况。

结果

在45例诊断为良性前列腺增生的患者中，1例（2%）尿沉渣中检测到GSTP1启动子高甲基化；7例前列腺上皮内瘤变患者中，2例（29%）检测到；22例早期包膜内癌患者中，15例（68%）检测到；18例局部晚期或全身性前列腺癌患者中，14例（78%）检测到。检测前列腺癌的特异性为98%，总体敏感性为73%。