Kobayashi H, Sato N, Kawamoto S, Saga T, Hiraga A, Ishimori T, Konishi J, Togashi K, Brechbiel M W
Hitachi Medical Co. chair, Department of Diagnostic and Interventional Imagiology, Kyoto University, Kyoto, Japan.
Magn Reson Med. 2001 Sep;46(3):579-85. doi: 10.1002/mrm.1230.
Noninvasive methods to visualize blood flow in the intratumoral vasculature have not previously been studied. In the present study, the use of a novel intravascular MR contrast agent with a generation-6 polyamidoamine dendrimer core (G6-(1B4M-Gd)192; MW: 175kD) was investigated, and the vasculature in experimental tumors was visualized using 3D MR angiography (MRA). Xenografted tumors in nude mice of two different histologies-KT005 (human osteogenic sarcoma) and LS180 (human colon carcinoma)-were used to obtain 3D MRA using G6-(1B4M-Gd)192 and Gd-DTPA. The contrast MR sectional images were correlated with the corresponding histological sections. The intratumoral vasculature in the KT005 tumor was clearly visualized by 3D MRA, which became more evident with the growth of the tumor xenograft. In contrast, the intratumoral vasculature in the LS180 tumor was sparser and much less developed than that in KT005 tumors. Blood vessels with a diameter as small as 100 microm based on histology were visualized using 0.033 mmol Gd/kg of G6-(1B4M-Gd)192. In conclusion, intratumoral vasculature with a 100-microm diameter was visualized better using 3D MRA with G6-(1B4M-Gd)192 than with Gd-DTPA.
此前尚未对可视化肿瘤内血管系统血流的非侵入性方法进行研究。在本研究中，对一种具有第6代聚酰胺-胺树枝状大分子核心（G6-(1B4M-Gd)192；分子量：175kD）的新型血管内磁共振造影剂的使用进行了研究，并使用三维磁共振血管造影（MRA）对实验性肿瘤的血管系统进行了可视化。使用G6-(1B4M-Gd)192和钆喷酸葡胺（Gd-DTPA）对两种不同组织学类型（KT005（人骨肉瘤）和LS180（人结肠癌））的裸鼠异种移植瘤进行三维MRA检查。将对比磁共振断层图像与相应的组织学切片进行关联。三维MRA清晰地显示了KT005肿瘤内的血管系统，随着异种移植瘤的生长，这一点变得更加明显。相比之下，LS¨80肿瘤内的血管系统比KT005肿瘤内的血管系统更稀疏且发育程度更低。基于组织学，使用0.033 mmol钆/千克的G6-(1B4M-Gd)192可显示直径小至100微米的血管。总之，与Gd-DTPA相比，使用G6-(1B4M-Gd)192进行三维MRA能更好地显示直径为100微米的肿瘤内血管系统。
