Ermolaeva S A, Tartakovskiĭ I S
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia.
Zh Mikrobiol Epidemiol Immunobiol. 2001 May-Jun(3):106-10.
The external and internal factors affecting the expression of L. monocytogenes virulence genes are analyzed. The role of protein PrfA, the only known factor directly affecting the transcription of virulence genes, as well as the contribution of other regulatory elements modulating the activity and/or intracellular concentration of PrfA are discussed. The environmental factors which influence the level of expression, such as temperature and the presence of metabolized carbohydrates in the cultivation medium, are analyzed. In addition, the possibility of autoregulating processes (the accumulation of L. monocytogenes-secreted autorepressor in the medium) is discussed and the possible mechanism of their action is suggested.
分析了影响单核细胞增生李斯特菌毒力基因表达的外部和内部因素。讨论了蛋白质PrfA（唯一已知直接影响毒力基因转录的因素）的作用，以及其他调节元件对PrfA活性和/或细胞内浓度调节的贡献。分析了影响表达水平的环境因素，如温度和培养基中代谢碳水化合物的存在。此外，还讨论了自调节过程（培养基中单核细胞增生李斯特菌分泌的自抑制因子的积累）的可能性，并提出了其可能的作用机制。
