有证据表明，单核细胞增生李斯特菌中毒力基因的多效激活因子PrfA可能存在但无活性。
Evidence that PrfA, the pleiotropic activator of virulence genes in Listeria monocytogenes, can be present but inactive.
作者信息
Renzoni A, Klarsfeld A, Dramsi S, Cossart P
机构信息
Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris, France.
出版信息
Infect Immun. 1997 Apr;65(4):1515-8. doi: 10.1128/iai.65.4.1515-1518.1997.
Abstract
All virulence genes of Listeria monocytogenes identified to date are positively regulated by PrfA, a transcriptional activator belonging to the Crp-Fnr family. Low temperature and cellobiose are two environmental signals known to repress expression of virulence genes in L. monocytogenes. In the present work, we analyzed the effect of temperature and cellobiose on the expression of the PrfA protein. At low temperature, PrfA was undetected, although prfA monocistronic transcripts are present. In contrast, PrfA was fully expressed in the presence of cellobiose. These results strongly suggest that virulence gene activation depends on both the presence of PrfA and additional regulatory pathways that either modify PrfA or act synergistically with PrfA.
摘要
迄今为止鉴定出的单核细胞增生李斯特菌的所有毒力基因均由PrfA正向调控，PrfA是一种属于Crp-Fnr家族的转录激活因子。低温和纤维二糖是已知可抑制单核细胞增生李斯特菌中毒力基因表达的两种环境信号。在本研究中，我们分析了温度和纤维二糖对PrfA蛋白表达的影响。在低温下，尽管存在prfA单顺反子转录本，但未检测到PrfA。相反，在纤维二糖存在的情况下，PrfA完全表达。这些结果有力地表明，毒力基因的激活既取决于PrfA的存在，也取决于其他可修饰PrfA或与PrfA协同作用的调控途径。
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