El-Awady M K, Gad Y Z, Wen Y, Eassawi M, Effat L, Amr K S, Ismail S, Christ G J
Human Genetics Department, National Research Center, Giza, Egypt.
World J Urol. 2001 Aug;19(4):263-6. doi: 10.1007/s003450100217.
Bladder carcinoma accounts for 26% of reported human malignancies in Egypt, and has been strongly associated with urinary schistosomiasis. Nevertheless, the immediate role of schistosomal egg proteins in bladder carcinogenesis is unexplored. We investigated the effects of crude soluble egg antigens (SEA) of Schistosoma hematobium on urothelial cell proliferation. The proliferation of bovine endothelial Endo, human urothelial J82 and smooth muscle SMC cell lines was assessed by low-density growth assays. SEA induced proliferation of both J82 and Endo cells in a dose-dependent fashion, but not SMC. Preboiling or proteinase K treatment of SEA abolished its effect. In addition, SEA enhanced urothelial expression of B-cell translocation protein (BTG1) and human proliferating cell nuclear antigen (PCNA) mRNAs. Given the strong correlation between cell proliferation and carcinogenesis, the findings suggest that crude SEA may play some role in schistosomal bladder carcinogenesis.
在埃及，膀胱癌占报告的人类恶性肿瘤的26%，并且一直与尿路血吸虫病密切相关。然而，血吸虫卵蛋白在膀胱癌发生中的直接作用尚未得到探索。我们研究了埃及血吸虫粗可溶性卵抗原（SEA）对尿路上皮细胞增殖的影响。通过低密度生长试验评估了牛内皮细胞Endo、人尿路上皮细胞J82和平滑肌细胞SMC细胞系的增殖情况。SEA以剂量依赖的方式诱导J82和Endo细胞的增殖，但不诱导SMC细胞增殖。对SEA进行预煮沸或蛋白酶K处理可消除其作用。此外，SEA增强了尿路上皮细胞中B细胞易位蛋白（BTG1）和人增殖细胞核抗原（PCNA）mRNA的表达。鉴于细胞增殖与癌症发生之间存在密切关联，这些发现表明粗SEA可能在血吸虫性膀胱癌发生中发挥一定作用。
