Tanaka N, Tajima S, Ishibashi A, Uchida K, Shigematsu T
Department of Dermatology, National Defense Medical College, Tokorozawa, Saitama, Japan.
Arch Dermatol Res. 2001 Jul;293(7):363-7. doi: 10.1007/s004030100239.
Acrolein and 4-hydroxy-2-nonenal (HNE) are both byproducts of a lipid peroxidation reaction. Actinic elastosis in photodamaged skin of aged individuals is characterized by the accumulation of fragmented elastic fibers in the sun-exposed areas. To study whether a lipid peroxidation reaction is involved in the accumulation of altered elastic fibers in actinic elastosis, skin specimens obtained from sun-damaged areas were immunohistochemically examined using the antibodies against acrolein and HNE. Both antibodies were found to react with the accumulations of elastic material. Double immunofluorescence labeling demonstrated that acrolein/elastin and HNE/elastin were colocalized in the actinic elastosis. Western blot analysis showed that the polypeptide with a molecular weight of 62 kDa reacted with anti-acrolein, anti-HNE and anti-elastin antibodies. The results suggest that acrolein and HNE may be associated with actinic elastosis.
丙烯醛和4-羟基-2-壬烯醛（HNE）都是脂质过氧化反应的副产物。老年个体光损伤皮肤中的光化性弹力纤维病的特征是在阳光暴露区域积聚破碎的弹性纤维。为了研究脂质过氧化反应是否参与光化性弹力纤维病中改变的弹性纤维的积聚，使用抗丙烯醛和抗HNE抗体对从阳光损伤区域获得的皮肤标本进行免疫组织化学检查。发现这两种抗体都与弹性物质的积聚发生反应。双重免疫荧光标记表明，丙烯醛/弹性蛋白和HNE/弹性蛋白在光化性弹力纤维病中共定位。蛋白质印迹分析表明，分子量为62 kDa的多肽与抗丙烯醛、抗HNE和抗弹性蛋白抗体发生反应。结果表明，丙烯醛和HNE可能与光化性弹力纤维病有关。
