Hellström S, Koslow S H
Acta Physiol Scand. 1975 Apr;93(4):540-7. doi: 10.1111/j.1748-1716.1975.tb05846.x.
A gas chromatographic-mass spectrometric method was used for the determination of biogenic amines in carotid body of adult and 10 days old rats. The method is ideally suited for this measurement since only small amounts of tissue were available (dry weight carotid body: adult 8.3 mug; infant 5.6 mug). In adult carotid body large amounts of dopamine (1 950 pmol/mg protein) and norepinephrine (1 140 pmol/mg protein) were found together with a comparatively small concentration of serotonin (505 pmol/mg protein). The carotid bodies of infant rats contained 1 065 pmol dopamine/mg protein and 410 pmol norepinephrine/mg protein. Epinephrine could not be detected. Surgical sympathetic denervation and chemical sympathectomy (6-hydroxydopamine) of adult carotid bodies did not significantly change the catecholamine content as compared to the controls. Reserpine depleted the catecholamines dosedependently. Administration of L-Dopa and pargyline (a monoamineoxidaseinhibitor) drastically increased the concentration of catecholamines. Treatment with a dopamine-beta-hydroxlase-inhibitor resulted in a decreased amount of norepinephrine without a simultaneous increase of dopamine. This may indicate that certain storage sites in this tissue may store dopamine while in other sites dopamine is a precursor of norepinephrine. Probably most of the dopamine and norepinephrine are stored in different cells.
采用气相色谱 - 质谱法测定成年大鼠和10日龄大鼠颈动脉体中的生物胺。该方法非常适合此项测量，因为可用的组织量很少（颈动脉体干重：成年大鼠8.3微克；幼鼠5.6微克）。在成年大鼠的颈动脉体中，发现大量多巴胺（1950皮摩尔/毫克蛋白质）和去甲肾上腺素（1140皮摩尔/毫克蛋白质），同时血清素浓度相对较低（505皮摩尔/毫克蛋白质）。幼鼠的颈动脉体含有1065皮摩尔多巴胺/毫克蛋白质和410皮摩尔去甲肾上腺素/毫克蛋白质。未检测到肾上腺素。与对照组相比，成年大鼠颈动脉体的手术交感神经去支配和化学交感神经切除术（6 - 羟基多巴胺）并未显著改变儿茶酚胺含量。利血平使儿茶酚胺含量呈剂量依赖性减少。给予左旋多巴和帕吉林（一种单胺氧化酶抑制剂）可显著增加儿茶酚胺浓度。用多巴胺 - β - 羟化酶抑制剂处理导致去甲肾上腺素含量减少，而多巴胺没有同时增加。这可能表明该组织中的某些储存位点可能储存多巴胺，而在其他位点多巴胺是去甲肾上腺素的前体。可能大部分多巴胺和去甲肾上腺素储存在不同的细胞中。
