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编码免疫原性二氢硫辛酰胺琥珀酰转移酶同源蛋白的布鲁氏菌羊种 sucB 基因的克隆、核苷酸序列及表达

Cloning, nucleotide sequence, and expression of the Brucella melitensis sucB gene coding for an immunogenic dihydrolipoamide succinyltransferase homologous protein.

作者信息

Zygmunt M S, Díaz M A, Teixeira-Gomes A P, Cloeckaert A

机构信息

Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France.

出版信息

Infect Immun. 2001 Oct;69(10):6537-40. doi: 10.1128/IAI.69.10.6537-6540.2001.

DOI:10.1128/IAI.69.10.6537-6540.2001
PMID:11553602
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC98793/
Abstract

The Brucella melitensis sucB gene encoding the dihydrolipoamide succinyltransferase (E2o) enzyme (previously identified as an immunogenic protein in infected sheep) was cloned and sequenced. The amino acid sequence predicted from the cloned gene revealed 88.8 and 51.2% identity to the dihydrolipoamide succinyltransferase SucB protein from Brucella abortus and Escherichia coli, respectively. Sera from naturally infected sheep showed antibody reactivity against the recombinant SucB protein.

摘要

编码二氢硫辛酰胺琥珀酰转移酶（E2o）（先前在受感染绵羊中被鉴定为一种免疫原性蛋白）的羊种布鲁氏菌sucB基因被克隆并测序。从克隆基因预测的氨基酸序列与流产布鲁氏菌和大肠杆菌的二氢硫辛酰胺琥珀酰转移酶SucB蛋白分别有88.8%和51.2%的同一性。自然感染绵羊的血清显示出对重组SucB蛋白的抗体反应性。

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本文引用的文献

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Microbiol Immunol. 1999;43(8):743-9. doi: 10.1111/j.1348-0421.1999.tb02465.x.
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The novel transmembrane Escherichia coli proteins involved in the amino acid efflux.参与氨基酸外排的新型跨膜大肠杆菌蛋白。
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Identification and characterization of Brucella ovis immunogenic proteins using two-dimensional electrophoresis and immunoblotting.利用二维电泳和免疫印迹法鉴定绵羊种布鲁氏菌免疫原性蛋白并进行特性分析。
Electrophoresis. 1997 Aug;18(8):1491-7. doi: 10.1002/elps.1150180824.
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Regulation of the TCA cycle and the general amino acid permease by overflow metabolism in Rhizobium leguminosarum.豆科根瘤菌中溢流代谢对三羧酸循环和通用氨基酸通透酶的调控
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Cloning, sequencing, and oxygen regulation of the Rhodobacter capsulatus alpha-ketoglutarate dehydrogenase operon.荚膜红细菌α-酮戊二酸脱氢酶操纵子的克隆、测序及氧调节
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