Mikami T, Saegusa M, Mitomi H, Yanagisawa N, Ichinoe M, Okayasu I
Dept of Pathology, School of Medicine, Kitasato University, 1-15-1 Kitasato, Sagamihara-si, Kanagawa, 228-8555, Japan.
Am J Clin Pathol. 2001 Sep;116(3):369-76. doi: 10.1309/VV6D-3GAH-VEJM-DUJT.
To clarify the relation between alteration of expression of cell adhesion molecules and progression of extrahepatic bile duct carcinomas. 55 cases were immunohistochemically examined for E-cadherin, alpha-catenin, beta-catenin, and CD44, with additional reverse transcription-polymerase chain reaction and Southern blotting hybridization (RT-PCR/SBH) assays. Levels of E-cadherin, alpha-catenin, and beta-catenin proteins were lower in carcinomas than in normal mucosa, while CD44 variants 3 and 6 were upregulated. Well-differentiated carcinoma showed higher expression of E-cadherin and alpha-catenin than moderately to poorly differentiated types. Macroscopically papillary lesions had higher expression of E-cadherin than their nonpapillary counterparts. RT-PCR/SBH for CD44 revealed the CD44 variant form to be more prevalent in carcinoma than in normal mucosa, correlating with the immunohistochemical results, and with more exon variety. The Cox proportional hazards test identified histologic type and E-cadherin expression as prognostic factors. Among the examined molecules, E-cadherin was especially related to papillary mass formation and a good prognosis.
为阐明细胞黏附分子表达改变与肝外胆管癌进展之间的关系。对55例患者的肝外胆管癌组织进行免疫组织化学检测，观察E-钙黏蛋白、α-连环蛋白、β-连环蛋白和CD44的表达情况，并采用逆转录聚合酶链反应和Southern印迹杂交（RT-PCR/SBH）检测。结果显示，癌组织中E-钙黏蛋白、α-连环蛋白和β-连环蛋白的水平低于正常黏膜，而CD44变体3和6表达上调。高分化癌组织中E-钙黏蛋白和α-连环蛋白的表达高于中低分化癌组织。大体上，乳头状病变中E-钙黏蛋白的表达高于非乳头状病变。CD44的RT-PCR/SBH检测显示，癌组织中CD44变体形式比正常黏膜中更普遍，与免疫组织化学结果相关，且外显子种类更多。Cox比例风险检验确定组织学类型和E-钙黏蛋白表达为预后因素。在所检测的分子中，E-钙黏蛋白尤其与乳头状肿块形成和良好预后相关。
