Kaplan D, Meyerson H, Lewandowska K
Dept of Pathology, Case Western Reserve University, Biomedical Research Bldg, Room 926, 2109 Adelbert Rd, Cleveland, OH 44106-4943, USA.
Am J Clin Pathol. 2001 Sep;116(3):429-36. doi: 10.1309/KXQ7-LHKC-CYQ8-R70W.
Immunophenotypic analysis of chronic lymphocytic leukemia (CLL) cells is essential for the diagnosis of this disorder. Unfortunately, surface immunoglobulin light chains and CD79b are expressed faintly or not at all by CLL cells from many patients. We developed an enzymatic amplification staining procedure that amplifies the fluorescent signal by 10- to 100-fold. By using this technology, we have been able to resolve immunoglobulin light chain exclusion and CD79b expression on the cells from most cases. This new capability can be used for high-resolution immunophenotypic analysis of leukemias and lymphomas.
慢性淋巴细胞白血病（CLL）细胞的免疫表型分析对于该疾病的诊断至关重要。不幸的是，许多患者的CLL细胞表面免疫球蛋白轻链和CD79b表达微弱或根本不表达。我们开发了一种酶促扩增染色程序，可将荧光信号放大10至100倍。通过使用这项技术，我们已经能够解析大多数病例细胞上的免疫球蛋白轻链排斥和CD79b表达情况。这种新能力可用于白血病和淋巴瘤的高分辨率免疫表型分析。
