Liang D C, Shih L Y, Kuo M C, Chai I J, Su T H, Chen S H, Liu H C, Shimosaka A
Department of Pediatrics, Mackay Memorial Hospital, 92, Sec. 2, Chung-San North Road, Taipei 104, Taiwan.
Pediatr Hematol Oncol. 2001 Sep;18(6):383-91. doi: 10.1080/088800101316922001.
The authors sought to determine whether recombinant human thrombopoietin (TPO) acts synergistically with other cytokines on burst-forming unit-erythroid (BFU-E)-derived and colony-forming unit-granulocyte/macrophage (CFU-GM)-derived colony formations from cord blood. Cord blood nonadherent mononuclear cells (MNC) from normal full-term neonates were cultured in a methylcellulose system. When cultured with 5 x 10(4) MNC/mL, erythropoietin (EPO) 2 U/mL, interleukin-3 (IL-3) 50 ng/mL, and/or TPO 400 ng/mL (experiment 1), the addition of TPO to EPO gave rise to more BFU-E-derived colonies (p = .002). The addition of TPO to EPO + IL-3 gave rise to more BFU-E-derived colonies (p = .006) also. TPO synergizes erythropoiesis from cord blood. When cultured with IL-3 50 ng/mL, granulocyte colony-stimulating factor (G-CSF) 25 ng/mL, and/or TPO 400 ng/mL, the addition of TPO to IL-3 gave rise to more CFU-GM-derived colonies (p = .002). The addition of TPO to G-CSF gave rise to more CFU-GM-derived colonies (p = .002) also. TPO synergizes myelopoiesis from cord blood. Thus, TPO has synergistic effects on both erythropoiesis and myelopoiesis from cord blood. In the identical conditions of culture, cord blood had significantly greater BFU-E-derived or CFU-GM-derived colony formation than bone marrow (in a previous report by the authors) did. When cultured under conditions similar to those of experiment 1, but with 1 x 10(4) cord blood MNC/mL and TPO 100 ng/mL (experiment 2), results similar to those in the experiment 1 also revealed that TPO has synergistic effects on erythropoiesis and myelopoiesis from cord blood. In every individual assay, the numbers of BFU-E-derived or CFU-GM-derived colonies in experiment 1 were significantly higher than those in experiment 2.
作者试图确定重组人血小板生成素（TPO）是否能与其他细胞因子协同作用，影响来自脐血的红系爆式集落形成单位（BFU-E）衍生的集落形成以及粒系/巨噬系集落形成单位（CFU-GM）衍生的集落形成。将正常足月新生儿的脐血非贴壁单核细胞（MNC）在甲基纤维素系统中培养。当以5×10⁴个MNC/mL、促红细胞生成素（EPO）2 U/mL、白细胞介素-3（IL-3）50 ng/mL和/或TPO 400 ng/mL进行培养时（实验1），在EPO中添加TPO可产生更多BFU-E衍生的集落（p = 0.002）。在EPO + IL-3中添加TPO也可产生更多BFU-E衍生的集落（p = 0.006）。TPO可协同促进脐血的红细胞生成。当以IL-3 50 ng/mL、粒细胞集落刺激因子（G-CSF）25 ng/mL和/或TPO 400 ng/mL进行培养时，在IL-3中添加TPO可产生更多CFU-GM衍生的集落（p = 0.002）。在G-CSF中添加TPO也可产生更多CFU-GM衍生的集落（p = 0.002）。TPO可协同促进脐血的髓系细胞生成。因此，TPO对脐血的红细胞生成和髓系细胞生成均具有协同作用。在相同的培养条件下，脐血产生的BFU-E衍生或CFU-GM衍生的集落形成明显多于（作者之前报告中的）骨髓。当在与实验1相似的条件下培养，但使用1×10⁴个脐血MNC/mL和TPO 100 ng/mL时（实验2），与实验1相似的结果也表明TPO对脐血的红细胞生成和髓系细胞生成具有协同作用。在每次单独检测中，实验1中BFU-E衍生或CFU-GM衍生的集落数量均显著高于实验2中的数量。
