Li C, Sun C Q, Mu P, Chen L, Wang X K
Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100094, China.
Yi Chuan Xue Bao. 2001 Aug;28(8):746-51.
A genetic map including 120 RFLP markers covering 1418.2 cM has been constructed using a BC1 population derived from a cross between common wild rice (Oryza rufipogon Griff.) from Dongxiang, Jiangxi Province, China, and an elite Indica variety Guichao 2. The linkage orders and genetic distances of markers in this map are consistent with the map constructed by Rice Genome Program of Japan except the markers of short arm of Chromosome 1. The QTLs analysis of anther length and ratio of stigma exsertion, two key traits of classification for cultivated rice and common wild rice indicated that there are two QTLs controlling anther length located at the region of C424-G39 of Chromosome 2, and C2807-C1263 of Chromosome 9 respectively. It was also found that there are two QTLs controlling ratio of stigma exsertion, and located at the region of C2289-R1553 of Chromosome 5 and G1149-R1963 of Chromosome 8 respectively. The detected QTLs affecting the key traits of classification for wild rice and cultivated rice will be useful for study of molecular evolution mechanism of cultivated rice.
利用来自中国江西省东乡的普通野生稻（东乡野生稻，Oryza rufipogon Griff.）与优良籼稻品种桂朝2杂交衍生的BC1群体，构建了一张包含120个RFLP标记、覆盖1418.2 cM的遗传图谱。该图谱中标记的连锁顺序和遗传距离与日本水稻基因组计划构建的图谱一致，除了第1染色体短臂上的标记。对栽培稻和普通野生稻分类的两个关键性状——花药长度和柱头外露率进行的QTL分析表明，有两个控制花药长度的QTL，分别位于第2染色体的C424 - G39区域和第9染色体的C2807 - C1263区域。还发现有两个控制柱头外露率的QTL，分别位于第5染色体的C2289 - R1553区域和第8染色体的G1149 - R1963区域。检测到的影响野生稻和栽培稻分类关键性状的QTL，将有助于栽培稻分子进化机制的研究。
