Shen Bo, Zhuang Jie-Yun, Zhang Ke-Qin, Xia Qi-Qing, Sheng Chen-Xia, Zheng Kang-Le
National Center for Rice Improvement, State Key Laboratory of Rice Biology, China National Rice Research Institute, Department of Life Science, Hangzhou Normal College, Hangzhou, China.
Yi Chuan Xue Bao. 2003 Dec;30(12):1133-9.
By employing a recombinant inbred line (RIL) population of 247 lines derived from an inidca-indica cross Zhenshan 97B x Milyang 46, a linkage map consisting of 158 DNA markers was constructed and used for the determination of QTLs conditioning five leaf traits and root exudates. The leaf traits analyzed were leaf area, leaf length, leaf width, leaf perimeter and leaf length/width ratio measured on top first leaf, top second and top third leaves. The RIL population showed transgressive segregation on each trait, and highly significant or significant positive correlations were observed between all traits except between leaf length and width, and between leaf perimeter and length/width ratio. A total of 24 QTLs located in 9 intervals were detected to have significant additive effects for leaf traits analyzed, with LOD scores ranging 2.9-11.8 and 4.0%-32.5% phenotypic variation explained for a single QTL. Clustering of QTLs for leaf traits was evident. In interval RM197-RZ516 on chromosome 6, 2 QTLs for leaf length, 2 QTLs for leaf width and 3 QTLs for leaf length/width ratio of different leaves were detected, among which the alleles for increasing trait values were from Zhenshan 97B for leaf length and leaf length/width ratio, and from Milyang 46 for leaf width. In interval RM1-RG532 on chromosome 1,2 QTLs for leaf length and 2 QTLs for leaf perimeter were detected on top first leaf and top second leaf, respectively, and all the alleles for increasing trait values were from Milyang 46. In this interval, larger additive effects were observed for QTLs detected on top first leaf than at top second leaf. In interval RZ667-B10B on chromosome 6, a QTL for leaf perimeter of top second leaf was detected, accounting for 8.0% phenotypic variation. In intervals RZ66-RM264 and RG81-RM313 on chromosome 8 and 12, each QTL was detected for leaf length of top third leaf and explained for 9.0% and 15.3% phenotypic variation, respectively. Epistasis analysis detected 56 and 4 significant additive-by-additive interactions for leaf traits and root vitality, explaining 2.7% to 13.7% and 6.8% to 14.9% of the total phenotypic variation, respectively. On comparison with QTLs for yield traits detected in the same population previously, it was found that the majority of QTLs for leaf traits and root vitality and those for yield traits were located in similar intervals. Fine mapping of QTLs for both leaf and yield traits in these chromosomal regions would facilitate investigations of the source-sink relationship in rice.
通过利用一个由247个株系组成的重组自交系（RIL）群体，该群体源自籼稻品种珍汕97B和密阳46的杂交，构建了一张包含158个DNA标记的连锁图谱，并用于定位调控5个叶片性状和根系分泌物的QTL。所分析的叶片性状包括顶叶第一叶、顶叶第二叶和顶叶第三叶的叶面积、叶长、叶宽、叶周长和叶长/宽比。RIL群体在每个性状上均表现出超亲分离，除叶长与叶宽、叶周长与叶长/宽比之间外，所有性状之间均观察到极显著或显著的正相关。共检测到位于9个区间的24个QTL对所分析的叶片性状具有显著的加性效应，LOD值范围为2.9 - 11.8，单个QTL解释的表型变异为4.0% - 32.5%。叶片性状的QTL聚类明显。在第6染色体的RM197 - RZ516区间，检测到不同叶片的叶长QTL 2个、叶宽QTL 2个和叶长/宽比QTL 3个，其中增加性状值的等位基因在叶长和叶长/宽比上来自珍汕97B，在叶宽上来自密阳46。在第1染色体的RM1 - RG532区间，分别在顶叶第一叶和顶叶第二叶上检测到叶长QTL 2个和叶周长QTL 2个，所有增加性状值的等位基因均来自密阳46。在该区间，顶叶第一叶上检测到的QTL比顶叶第二叶上的QTL具有更大加性效应。在第6染色体的RZ667 - B10B区间，检测到顶叶第二叶叶周长的一个QTL，解释表型变异的8.0%。在第8和12染色体的RZ66 - RM264和RG81 - RM313区间，分别检测到顶叶第三叶叶长的QTL，解释表型变异的9.0%和15.3%。上位性分析检测到叶片性状和根系活力的加性 - 加性互作分别有56个和4个显著互作，分别解释总表型变异的2.7% - 13.7%和6.8% - 14.9%。与之前在同一群体中检测到的产量性状QTL相比，发现叶片性状和根系活力的大多数QTL与产量性状的QTL位于相似区间。对这些染色体区域中叶片和产量性状QTL的精细定位将有助于研究水稻的源 - 库关系。
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