Yu L, Niu J S, Ma Z Q, Li X Y, Chen P D, Liu D J
Key Laboratory of Crop Cytogenetics, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
Yi Chuan Xue Bao. 2001 Aug;28(8):760-8.
A Triticum aestivum-Haynaldia villosa 6VS/6AL translocation line contained powdery mildew resistance gene Pm21 which is effective against all the current biotypes of Erygsiphe gramins. Cloning of the related genes for powdery mildew resistance is significance for understanding its resistance mechanism and disease resistance breeding. Using RT-PCR and RACE technology, a wheat pathogenesis related protein 1 cDNA clone (TaPr-1) corresponding to a mRNA differentially induced in resistant 6VS/6AL translocation line compared to susceptible wheat cultivar "Yangmai 5" by powdery mildew infection was isolated and characterized. This sequence contained 823 bp and had an open reading frame (ORF) containing 164 amino acids with 24 amino acids in the putative signal peptide and 140 amino acids comprising the mature peptide (15.1 kD). The deduced amino acid sequence showed close homology to PR-1 like proteins, which have been isolated from many plants. Northern blot analysis revealed the most abundantly accumulation of the corresponding mRNA 12 h after infection in translocation line (6VS/6AL). The obviously difference in the expression of the PRw-1 was also observed between resistant translocation line (6VS/6AL) and susceptible parent "Yangmai 5", it showed the TaPr-1 gene is related to powdery mildew resistance. Southern blot indicated that the wheat genome contains more than one copies of TaPr-1 genes, and there are polymorphism between translocation lines and "Yanmai 5", the result shows that 6VS may contain TaPr-1 genes.
一个普通小麦-簇毛麦6VS/6AL易位系含有抗白粉病基因Pm21，该基因对当前所有小麦白粉菌生理小种均有效。克隆白粉病抗性相关基因对于理解其抗性机制和抗病育种具有重要意义。利用RT-PCR和RACE技术，分离并鉴定了一个与小麦病程相关蛋白1 cDNA克隆（TaPr-1），该克隆对应于抗白粉病的6VS/6AL易位系与感病小麦品种“扬麦5号”相比，受白粉菌侵染后差异诱导的mRNA。该序列包含823 bp，有一个开放阅读框（ORF），包含164个氨基酸，其中推定信号肽有24个氨基酸，成熟肽包含140个氨基酸（15.1 kD）。推导的氨基酸序列与从许多植物中分离出的PR-1类蛋白具有高度同源性。Northern杂交分析显示，在易位系（6VS/6AL）中，感染后12 h相应mRNA积累最为丰富。在抗白粉病易位系（6VS/6AL）和感病亲本“扬麦5号”之间也观察到PRw-1表达的明显差异，表明TaPr-1基因与白粉病抗性相关。Southern杂交表明，小麦基因组含有多个TaPr-1基因拷贝，易位系与“扬麦5号”之间存在多态性，结果表明6VS可能含有TaPr-1基因。
