da Motta P G, de Figueiredo C B, Maltos S M, Nicoli J R, Ribeiro Sobrinho A P, Maltos K L, Carvalhais H P
Dental School, Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Int Endod J. 2001 Sep;34(6):435-9. doi: 10.1046/j.1365-2591.2001.00412.x.
The objective of the present study was to assess the efficacy of 2.5% sodium hypochlorite and 2.2% glutaraldehyde ('Cidex') as sterilizing agents for gutta-percha cones. The efficacy of storage of gutta-percha cones in the presence or absence of paraformaldehyde was also evaluated.
Gutta-percha cones artificially contaminated with a suspension of Bacillus stearothermophilus (ATCC/7953) were treated with either 2.2% glutaraldehyde for 10, 15, 30 and 60 min and 10 and 12 h, or 2.5% sodium hypochlorite for 5, 10 and 15 min. The cones were then incubated in thioglycollate medium for the determination of microbial growth. In parallel, additional sterile gutta-percha cones were stored in sealed containers with or without paraformaldehyde tablets for 30 days. The containers were opened 30 min a day and exposed to the environment of a functioning dental clinic. Twelve cones were removed weekly from the containers to determine whether contamination had occurred.
The results showed that 2.5% sodium hypochlorite was effective after 5, 10 and 15 min, whereas 10 and 12 h contact with 2.2% glutaraldehyde was necessary to obtain sterilization. There was no contamination of the gutta-percha cones when stored with or without paraformaldehyde.
Sodium hypochlorite (2.5%) and 2.2% glutaraldehyde ('Cidex') proved to be effective as sterilizing agents for gutta-percha cones, with sodium hypochlorite requiring shorter periods of use. No difference was observed between the two methods of cone storage.
本研究的目的是评估2.5%次氯酸钠和2.2%戊二醛（“Cidex”）作为牙胶尖消毒灭菌剂的效果。同时还评估了在有或没有多聚甲醛存在的情况下储存牙胶尖的效果。
用嗜热脂肪芽孢杆菌（ATCC/7953）悬浮液人工污染的牙胶尖，分别用2.2%戊二醛处理10、15、30和60分钟以及10和12小时，或用2.5%次氯酸钠处理5、10和15分钟。然后将牙胶尖在硫乙醇酸盐培养基中培养以测定微生物生长情况。同时，将另外的无菌牙胶尖存放在有或没有多聚甲醛片的密封容器中30天。每天打开容器30分钟并暴露于正常运转的牙科诊所环境中。每周从容器中取出12个牙胶尖以确定是否发生污染。
结果表明，2.5%次氯酸钠在处理5、10和15分钟后有效，而与2.2%戊二醛接触10和12小时才能达到灭菌效果。无论是否与多聚甲醛一起储存，牙胶尖均未被污染。
2.5%次氯酸钠和2.2%戊二醛（“Cidex”）被证明是有效的牙胶尖消毒灭菌剂，次氯酸钠所需的处理时间更短。两种牙胶尖储存方法之间未观察到差异。
