Evaluation of surface analysis of gutta-percha after disinfecting with sodium hypochlorite, silver nanoparticles, and chitosan nanoparticles by atomic force microscopy: An study.

AIMS AND OBJECTIVES

The purpose of this study is to evaluate the surface topography of gutta-percha (GP) after disinfecting with sodium hypochlorite (NaOCl) (5.25%), silver nanoparticles (AgNPs) (70 µg/ml), and chitosan nanoparticles (ChNPs) (1.5 mg/ml) by atomic force microscopy (AFM).

MATERIALS AND METHODS

Forty GP cones were taken in this study. These samples were divided into four different groups such as Group I - control group (untreated GP Points) and Group II, III, and IV were treated with 5.25% NaOCl, 70 µg/ml AgNPs, and 1.5 mg/ml ChNPs, respectively. The surface topography analysis of the samples was performed using AFM.

STATISTICAL ANALYSIS

Root mean square (RMS) and surface roughness parameters were used to compare the structure of GP points with contact mode imaging. These values were tested by IBM SPSS-20.0 version statistical software using one-way ANOVA and (Tukey's honestly significant difference) tests. They were considered statistically significant when < 0.05.

RESULTS

The RMS and surface roughness values are significantly higher for NaOCl group (5.25% NaOCl) when compared with AgNPs group (70 µg/ml AgNPs) and ChNPs group (1.5 mg/ml ChNPs).

CONCLUSION

This study has shown more surface topography deterioration of GP treated with NaOCl and lesser deterioration with AgNPs and ChNPs which affects postoperative prognosis.