Parsons Y N, Glendinning K J, Thornton V, Hales B A, Hart C A, Winstanley C
Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Duncan Building, Daulby Street, Liverpool L69 3GA, UK.
FEMS Microbiol Lett. 2001 Sep 11;203(1):103-8. doi: 10.1111/j.1574-6968.2001.tb10827.x.
Using probes constructed from Ralstonia solanacearum and Burkholderia pseudomallei, putative type III secretion (TTS) genes were identified in Burkholderia cepacia J2315 (genomovar III). A cosmid clone containing DNA with homology to five TTS genes was sub-cloned and regions were sequenced in order to design oligonucleotides for polymerase chain reaction assays. These indicated that two putative TTS genes (bcscQ and bcscV) were present in all members of the B. cepacia complex with the exception of strains from genomovar I. Southern blot assays confirmed this observation, suggesting that the lack of a TTS gene cluster may define a major difference between B. cepacia genomovar I and other members of the B. cepacia complex, including genomovar III. In contrast to TTS gene clusters in other bacteria, a putative gene homologous to the virB1 gene of Brucella suis was located directly downstream of bcscQR.
利用由青枯雷尔氏菌（Ralstonia solanacearum）和类鼻疽伯克霍尔德菌（Burkholderia pseudomallei）构建的探针，在洋葱伯克霍尔德菌J2315（基因变种III）中鉴定出了假定的III型分泌（TTS）基因。将一个含有与五个TTS基因具有同源性的DNA的黏粒克隆进行亚克隆，并对各区域进行测序，以便设计用于聚合酶链反应分析的寡核苷酸。这些结果表明，除了来自基因变种I的菌株外，洋葱伯克霍尔德菌复合体的所有成员中都存在两个假定的TTS基因（bcscQ和bcscV）。Southern杂交分析证实了这一观察结果，表明缺乏TTS基因簇可能是洋葱伯克霍尔德菌基因变种I与洋葱伯克霍尔德菌复合体的其他成员（包括基因变种III）之间的一个主要差异。与其他细菌中的TTS基因簇不同，一个与猪布鲁氏菌（Brucella suis）的virB1基因同源的假定基因直接位于bcscQR的下游。
