Vandamme P, Mahenthiralingam E, Holmes B, Coenye T, Hoste B, De Vos P, Henry D, Speert D P
Laboratorium voor Microbiologie, Faculteit Wetenschappen, Universiteit Gent, Ghent, Belgium.
J Clin Microbiol. 2000 Mar;38(3):1042-7. doi: 10.1128/JCM.38.3.1042-1047.2000.
The Burkholderia cepacia complex currently comprises five genomic species, i.e., B. cepacia genomovar I, B. multivorans (formerly known as B. cepacia genomovar II), B. cepacia genomovar III, B. cepacia genomovar IV, and B. vietnamiensis (also known as B. cepacia genomovar V). In the absence of straightforward diagnostic tests for the identification of B. cepacia genomovars I, III, and IV, the last two genomic species were not formally classified as novel Burkholderia species (genomovar I contains the type strain and therefore retains the name B. cepacia). In the present study, we describe differential biochemical tests and a recA gene-based PCR assay for the routine identification of strains currently known as B. cepacia genomovar IV and propose formal classification of this organism as Burkholderia stabilis sp. nov. B. stabilis can indeed be differentiated from all other B. cepacia complex strains by the absence of beta-galactosidase activity, from strains of B. cepacia genomovars I and III and B. vietnamiensis by the inability to oxidize sucrose, and from B. multivorans by the lack of growth at 42 degrees C. In addition, analysis with the recA gene-derived primers BCRG41 (5'-ACCGGCGAGCAGGCGCTT-3') and BCRG42 (5'-ACGCCATCGGGCATGGCA-3') specifically allows the detection of B. stabilis strains in a conventional PCR assay. Examination of a set of 21 B. stabilis strains by means of random amplified polymorphic DNA analysis and pulsed-field gel electrophoresis typing suggested that the genome of this organism is highly conserved, which is in sharp contrast to the generally accepted genomic diversity, variability, and plasticity among B. cepacia strains.
洋葱伯克霍尔德菌复合体目前包含五个基因组种，即洋葱伯克霍尔德菌基因组I型、多食伯克霍尔德菌（以前称为洋葱伯克霍尔德菌基因组II型）、洋葱伯克霍尔德菌基因组III型、洋葱伯克霍尔德菌基因组IV型和越南伯克霍尔德菌（也称为洋葱伯克霍尔德菌基因组V型）。由于缺乏用于鉴定洋葱伯克霍尔德菌基因组I型、III型和IV型的直接诊断测试，后两个基因组种未被正式分类为新的伯克霍尔德菌物种（基因组I型包含模式菌株，因此保留洋葱伯克霍尔德菌这个名称）。在本研究中，我们描述了用于常规鉴定目前称为洋葱伯克霍尔德菌基因组IV型菌株的鉴别生化试验和基于recA基因的PCR检测方法，并提议将该菌正式分类为稳定伯克霍尔德菌新种。稳定伯克霍尔德菌确实可以通过缺乏β-半乳糖苷酶活性与所有其他洋葱伯克霍尔德菌复合体菌株区分开来，通过不能氧化蔗糖与洋葱伯克霍尔德菌基因组I型、III型菌株以及越南伯克霍尔德菌区分开来，通过在42℃不能生长与多食伯克霍尔德菌区分开来。此外，使用源自recA基因的引物BCRG41（5'-ACCGGCGAGCAGGCGCTT-3'）和BCRG42（5'-ACGCCATCGGGCATGGCA-3'）进行分析，能够在常规PCR检测中特异性地检测到稳定伯克霍尔德菌菌株。通过随机扩增多态性DNA分析和脉冲场凝胶电泳分型对一组21株稳定伯克霍尔德菌菌株进行检测，结果表明该菌的基因组高度保守，这与普遍认为的洋葱伯克霍尔德菌菌株间的基因组多样性、变异性和可塑性形成鲜明对比。
