Baylin S B, Mendelsohn G, Weisburger W R, Gann D S, Eggleston J C
Cancer. 1979 Oct;44(4):1315-21. doi: 10.1002/1097-0142(197910)44:4<1315::aid-cncr2820440423>3.0.co;2-1.
The distribution of calcitonin, L-dopa decarboxylase, and histaminase is studied in sectioned total thyroid glands from patients with different stages of familial medullary thyroid carcinoma. In 5 glands with gross carcinoma and in 3 with early microscopic carcinoma the distribution of all three parameters positively correlates (p less than .01 for each correlation). In contrast, in 6 glands with C-cell hyperplasia only the distribution of calcitonin and L-dopa decarboxylase correlates (r = 0.64, p less than .01) while those for histaminase vs. calcitonin (r = .17, p = N.S.) and histaminase vs. dopa decarboxylase (r = .03, p = N.S.) do not. In the glands with microscopic carcinoma the peak levels of histaminase occur in the areas of disease as defined by immunohistochemical staining of calcitonin; mean histaminase activity is the only one of the three parameters measured that distinguishes between C-cell hyperplasia and microscopic carcinoma (p less than .005). Immunohistochemical staining of histaminase shows positive cells in glands with gross and microscopic carcinoma, but in none of the glands with hyperplasia alone. Histaminase is thus found in high amounts in some malignant C-cells only and may be a useful marker to distinguish between hyperplasia and malignancy in thyroids with early C-cell proliferative disorders.
对患有不同阶段家族性甲状腺髓样癌患者的全甲状腺切片进行降钙素、L-多巴脱羧酶和组胺酶分布的研究。在5个有肉眼可见癌的腺体和3个有早期微小癌的腺体中，所有这三个参数的分布呈正相关（每种相关性的p值均小于0.01）。相比之下，在6个有C细胞增生的腺体中，只有降钙素和L-多巴脱羧酶的分布相关（r = 0.64，p小于0.01），而组胺酶与降钙素（r = 0.17，p =无显著性差异）以及组胺酶与多巴脱羧酶（r = 0.03，p =无显著性差异）的分布不相关。在微小癌的腺体中，组胺酶的峰值水平出现在通过降钙素免疫组化染色定义的病变区域；平均组胺酶活性是所测量的三个参数中唯一能区分C细胞增生和微小癌的参数（p小于0.005）。组胺酶的免疫组化染色显示，在有肉眼可见癌和微小癌的腺体中有阳性细胞，但在仅伴有增生的腺体中没有。因此，组胺酶仅在一些恶性C细胞中大量存在，可能是区分早期C细胞增殖性疾病甲状腺增生和恶性病变的有用标志物。
