Abeş M, Sarihan H, Değer O, Uydu H A
Department of Pediatric Surgery and Biochemistry, Karadeniz Technical University, Faculty of Medicine, Trabzon, Turkey.
Eur J Pediatr Surg. 2001 Aug;11(4):255-8. doi: 10.1055/s-2001-17146.
This study was designed to investigate the effect of ATP-MgCl(2) administered before and after detorsion on the prevention of reperfusion injury after unilateral testicular torsion. The rats were divided into six groups, each containing six rats. Torsion was created by rotating the left testes 720 degrees in a clockwise direction. Group 1 functioned as a control group. Torsion only was carried out in Group 2. Detorsion was carried out in Group 3. ATP-MgCl(2) (100 micromol/kg) was injected intravenously immediately before detorsion in Group 4. ATP-MgCl(2) (100 micromol/kg) was injected intravenously immediately after detorsion in Group 5. Saline was injected intravenously immediately after detorsion in Group 6. The effect of ATP-MgCl(2) on reperfusion injury was investigated by determining the levels of thiobarbituric acid-reactive substances (TBAR) and resulting lipid peroxidation in the bilateral testicular tissue. Testicular torsion and detorsion caused a significant increase in the TBAR levels in the bilateral testicular tissue. TBAR levels decreased to approximately normal levels in Groups 4 and 5. It is concluded that if reperfusion injury has occurred in both testes after unilateral testicular torsion, ATP-MgCl(2) administered before or after detorsion may prevent reperfusion injury in testicular torsion.
本研究旨在探讨在单侧睾丸扭转复位前后给予ATP-MgCl₂对预防再灌注损伤的作用。将大鼠分为六组，每组六只。通过将左侧睾丸顺时针旋转720度造成扭转。第1组作为对照组。第2组仅进行扭转。第3组进行复位。第4组在复位前立即静脉注射ATP-MgCl₂（100微摩尔/千克）。第5组在复位后立即静脉注射ATP-MgCl₂（100微摩尔/千克）。第6组在复位后立即静脉注射生理盐水。通过测定双侧睾丸组织中硫代巴比妥酸反应性物质（TBAR）水平及由此产生的脂质过氧化反应，研究ATP-MgCl₂对再灌注损伤的影响。睾丸扭转和复位导致双侧睾丸组织中TBAR水平显著升高。第4组和第5组的TBAR水平降至大致正常水平。得出结论：如果单侧睾丸扭转后双侧睾丸均发生了再灌注损伤，在复位前后给予ATP-MgCl₂可能预防睾丸扭转中的再灌注损伤。
