Department of Surgery, Zhejiang Medical College, Hangzhou City, Zhejiang Province, 310053, China.
J Pediatr Surg. 2011 Jul;46(7):1419-24. doi: 10.1016/j.jpedsurg.2010.09.044.
The pathophysiology of testicular torsion-detorsion is an ischemia-reperfusion injury caused by overgeneration of reactive oxygen species (ROS). This study aimed to investigate the effect of rutin, a well-known antioxidant, on testicular ischemia-reperfusion injury.
Sixty male Sprague-Dawley rats were randomly divided into 3 groups, each containing 20 rats. Rats in the control group underwent a sham operation of the left testis. In the torsion-detorsion group, the left testis was rotated 720° for 2 hours. Rats in the treatment group received the same surgical procedure as the torsion-detorsion group, but rutin was administered intravenously at the time of detorsion. Bilateral orchiectomy was performed on half of the rats in each experimental group at 4 hours after detorsion for measurement of malondialdehyde, an indicator of intratesticular ROS content, and for evaluation of superoxide dismutase and catalase, which are endogenous antioxidant enzymes. Orchiectomy was performed on the remaining rats at 3 months after detorsion for analysis of testicular spermatogenesis.
Unilateral testicular torsion-detorsion caused a significant increase in malondialdehyde level and caused significant decreases in superoxide dismutase, catalase activities, and spermatogenesis in ipsilateral testes. The rats treated with rutin had a significant decrease in malondialdehyde level and had significant increases in superoxide dismutase, catalase activities, and spermatogenesis in ipsilateral testes, compared with torsion-detorsion group.
Rutin protects testes from ischemia-reperfusion injury. The protective effect of rutin may be caused by scavenging ROS by increasing superoxide dismutase and catalase activities.
睾丸扭转-复位的病理生理学是由活性氧（ROS）过度生成引起的缺血再灌注损伤。本研究旨在探讨芦丁（一种众所周知的抗氧化剂）对睾丸缺血再灌注损伤的影响。
将 60 只雄性 Sprague-Dawley 大鼠随机分为 3 组，每组 20 只大鼠。对照组大鼠行左侧睾丸假手术。在扭转-复位组，左侧睾丸旋转 720°2 小时。治疗组大鼠接受与扭转-复位组相同的手术程序，但在复位时静脉给予芦丁。每组一半的大鼠在复位后 4 小时行双侧睾丸切除术，以测量丙二醛（一种睾丸内 ROS 含量的指标），并评估超氧化物歧化酶和过氧化氢酶，这两种酶都是内源性抗氧化酶。其余大鼠在复位后 3 个月行双侧睾丸切除术，以分析睾丸生精情况。
单侧睾丸扭转-复位导致丙二醛水平显著升高，并导致对侧睾丸中超氧化物歧化酶、过氧化氢酶活性和生精功能显著降低。与扭转-复位组相比，芦丁治疗组大鼠丙二醛水平显著降低，超氧化物歧化酶、过氧化氢酶活性和对侧睾丸生精功能显著增加。
芦丁可保护睾丸免受缺血再灌注损伤。芦丁的保护作用可能是通过增加超氧化物歧化酶和过氧化氢酶的活性来清除 ROS 引起的。
