Ivanova E G, Doronina N V, Trotsenko Iu A
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow Oblast, 142290 Russia.
Mikrobiologiia. 2001 Jul-Aug;70(4):452-8.
Obligately and facultatively methylotrophic bacteria with different pathways of C1 metabolism were found to be able to produce auxins, particularly indole-3-acetic acid (IAA), in amounts of 3-100 micrograms/ml. Indole-3-pyruvic acid and indole-3-acetamide were detected only in methylobacteria with the serine pathway of C1 metabolism, Methylobacterium mesophilicum and Aminobacter aminovorans. The production of auxins by methylobacteria was stimulated by the addition of tryptophan to the growth medium and was inhibited by ammonium ions. The methylobacteria under study lacked tryptophan decarboxylase and tryptophan side-chain oxidase. At the same time, they were found to contain several aminotransferases. IAA is presumably synthesized by methylobacteria through indole-3-pyruvic acid.
已发现具有不同一碳代谢途径的专性和兼性甲基营养型细菌能够产生生长素,特别是吲哚 - 3 - 乙酸(IAA),产量为3 - 100微克/毫升。仅在具有一碳代谢丝氨酸途径的甲基细菌、嗜温甲基杆菌和氨基营养氨基杆菌中检测到吲哚 - 3 - 丙酮酸和吲哚 - 3 - 乙酰胺。向生长培养基中添加色氨酸可刺激甲基细菌产生生长素,而铵离子则对其有抑制作用。所研究的甲基细菌缺乏色氨酸脱羧酶和色氨酸侧链氧化酶。与此同时,发现它们含有几种转氨酶。IAA可能是甲基细菌通过吲哚 - 3 - 丙酮酸合成的。