Sasaki T, Igarashi Y, Saito N, Furumai T
Biotechnology Research Center, Toyama Prefectural University, Kosugi, Japan.
J Antibiot (Tokyo). 2001 Jul;54(7):567-72. doi: 10.7164/antibiotics.54.567.
Two novel butyrolactone antibiotics, cedarmycins A and B, were isolated from the cultured broth of the actinomycete Streptomyces sp. TP-A0456. The new compounds were purified by HP-20 resin, silica gel, ODS column chromatographies and preparative HPLC, consecutively. The structure of cedarmycin was determined by spectroscopic methods as an alpha,beta-unsaturated butyrolactone with a fatty acid side chain. These compounds showed antibiotic activity against Gram-positive and -negative bacteria and yeasts. Among the tested organisms, cedarmycins potently inhibited the growth of Candida glabrata IFO 0622 with the MIC of 0.4 microg/ml, comparable to that of amphotericin B.
从链霉菌属菌株Streptomyces sp. TP-A0456的培养液中分离出两种新型丁内酯抗生素，雪松霉素A和B。新化合物依次通过HP-20树脂、硅胶、ODS柱色谱和制备型高效液相色谱进行纯化。通过光谱方法确定雪松霉素的结构为具有脂肪酸侧链的α,β-不饱和丁内酯。这些化合物对革兰氏阳性菌、革兰氏阴性菌和酵母显示出抗菌活性。在所测试的微生物中，雪松霉素能有效抑制光滑念珠菌IFO 0622的生长，其最低抑菌浓度为0.4微克/毫升，与两性霉素B相当。
