Setiawan V W, Zhang Z F, Yu G P, Lu Q Y, Li Y L, Lu M L, Wang M R, Guo C H, Yu S Z, Kurtz R C, Hsieh C C
Department of Epidemiology, UCLA School of Public Health, and Jonsson Comprehensive Cancer Center, Los Angeles, CA 90095, USA.
Cancer Causes Control. 2001 Oct;12(8):673-81. doi: 10.1023/a:1011261602940.
In a population-based case-control study in Yangzhong, China, we investigated the relationship between genetic polymorphisms of GSTP1 and susceptibility to gastric cancer and its premalignant lesion, chronic gastritis. The possible gene-gene interactions between GSTP1 polymorphisms and GSTM1, GSTT1 genes were explored.
Epidemiologic data were collected by standard questionnaire from 133 gastric cancer cases, 166 chronic gastritis cases, and 433 cancer-free population controls. Blood samples for Helicobacter pylori and molecular marker assays were collected from 84 gastric cancer cases, 146 chronic gastritis, and 429 population controls. GSTP1 polymorphisms were determined by the PCR-RFLP method and H. pylori infection was measured by the ELISA method. Associations between certain GSTP1 genotypes and both gastric cancer and chronic gastritis were assessed by odds ratios (ORs) and 95% confidence intervals (CIs) derived from logistic regression.
The distributions of three GSTP1 genotypes, Ile/Ile, Ile/Val, and Val/Val, were similar in gastric cancer cases, chronic gastritis, and controls. After adjusting for age, gender, education, body mass index, pack-year of smoking, alcohol drinking, H. pylori infection, salt and fruit intakes, the adjusted ORs of Val/Val were 1.3 (95% CI: 0.1-11.2) for gastric cancer and 0.9 (95% CI: 0.2-4.8) for chronic gastritis. Combining the Val alleles (Val/Val and Ile/Val) into one group, no association was observed between GSTP1 and both gastric cancer and chronic gastritis. In addition, the allelism at the GSTP1 locus did not increase gastric cancer and chronic gastritis risks associated with the GSTM1 or GSTT1 genotypes.
Our data suggest that the GSTP1 genotype seems not to be associated with the risk of gastric cancer and chronic gastritis in a high-risk Chinese population.
在中国扬中进行的一项基于人群的病例对照研究中，我们调查了GSTP1基因多态性与胃癌及其癌前病变慢性胃炎易感性之间的关系。探讨了GSTP1基因多态性与GSTM1、GSTT1基因之间可能存在的基因-基因相互作用。
通过标准问卷收集了133例胃癌病例、166例慢性胃炎病例和433例无癌人群对照的流行病学数据。从84例胃癌病例、146例慢性胃炎病例和429例人群对照中采集用于幽门螺杆菌和分子标志物检测的血样。采用PCR-RFLP方法确定GSTP1基因多态性，采用ELISA方法检测幽门螺杆菌感染情况。通过逻辑回归得出的比值比（OR）和95%置信区间（CI）评估特定GSTP1基因型与胃癌和慢性胃炎之间的关联。
Ile/Ile、Ile/Val和Val/Val这三种GSTP1基因型在胃癌病例、慢性胃炎病例和对照中的分布相似。在调整年龄、性别、教育程度、体重指数、吸烟包年数、饮酒、幽门螺杆菌感染、盐和水果摄入量后，Val/Val基因型对于胃癌的校正OR为1.3（95%CI：0.1-11.2），对于慢性胃炎的校正OR为0.9（95%CI：0.2-4.8）。将Val等位基因（Val/Val和Ile/Val）合并为一组后，未观察到GSTP1与胃癌和慢性胃炎之间存在关联。此外，GSTP1基因座的等位基因状态并未增加与GSTM1或GSTT1基因型相关的胃癌和慢性胃炎风险。
我们的数据表明，在高危中国人群中，GSTP1基因型似乎与胃癌和慢性胃炎风险无关。
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