与肽核酸（PNA）相关的寡核苷酸模拟物及其在核酸杂交研究与分析中的评估。

PNA-related oligonucleotide mimics and their evaluation for nucleic acid hybridization studies and analysis.

作者信息

Efimov V, Choob M, Buryakova A, Phelan D, Chakhmakhcheva O

机构信息

Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, 117871 Moscow, Russia.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):419-28. doi: 10.1081/NCN-100002316.

DOI:10.1081/NCN-100002316

PMID:11563057

Abstract

DNA mimics containing phosphonate analogues of PNAs (pPNAs), particularly PNA-pPNA hybrids as well as hetero-oligomers consisted of pPNA units and PNA-like molecules on the base of trans-4-hydroxy-L-proline (HypNA) have been synthesized. The evaluation of their effectiveness in assays based on the hybridization technique in the comparison with natural oligonucleotides and classical PNAs has shown a high potential of these mimics as sensor molecules for nucleic acid based diagnostics and as molecular probes for mRNA isolation.

摘要

已合成了包含肽核酸（PNA）的膦酸酯类似物的DNA模拟物（pPNA），特别是PNA-pPNA杂交体以及由pPNA单元和基于反式-4-羟基-L-脯氨酸（HypNA）的PNA样分子组成的杂合寡聚物。与天然寡核苷酸和经典PNA相比，在基于杂交技术的检测中对它们的有效性评估表明，这些模拟物作为基于核酸的诊断传感器分子和mRNA分离的分子探针具有很高的潜力。