Chernolovskaya E L, Koshkin A A, Vlassov V V
Novosibirsk Institute of Bioorganic Chemistry, Lavrentiev ave., 8, Novosibirsk, 630090, Russia.
Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):847-50. doi: 10.1081/NCN-100002443.
LNA oligonucleotides [1] can be used for targeting to double stranded DNA by the "strand invasion" mechanism. We used affinity modification by reactive oligonucleotide conjugates for investigation of oligonucleotides interaction with structured DNA. The tested LNAs and oligonucleotides of the same sequence were assayed as anti-mdr1 drugs in different cell cultures. One of the oligos, LNA79 strongly inhibited mdr1 induction in Hela cells and totally prevented activation of mdr1 in K-562.
锁核酸（LNA）寡核苷酸[1]可通过“链侵入”机制靶向双链DNA。我们使用反应性寡核苷酸偶联物进行亲和修饰，以研究寡核苷酸与结构化DNA的相互作用。将相同序列的受试锁核酸和寡核苷酸作为抗多药耐药1（mdr1）药物在不同细胞培养物中进行测定。其中一种寡核苷酸LNA79强烈抑制了人宫颈癌（Hela）细胞中mdr1的诱导，并完全阻止了人慢性髓原白血病细胞（K-562）中mdr1的激活。
