用于掺入寡聚RNA的D-[1'-13C]-核糖核苷的高效合成。
Efficient synthesis of D-[1'-13C]-ribonucleosides for incorporation into oligo-RNA.
作者信息
Saito Y, Nyilas A, Agrofoglio L A
机构信息
I.C.O.A. associé CNRS, Faculté des Sciences, 45100 Orléans-France.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):937-40. doi: 10.1081/NCN-100002463.
PMID:11563149
Abstract
Syntheses of the monomer building blocks used for the solid-phase synthesis of specifically 1'-13C-labeled oligoribonucleotides from the D-[1-13C]ribose is presented. Procedure has been used for the selective formation of 2'-O-silylated ribonucleosides. After 5'-O-dimethoxytritylation, the synthesis of D-[1'-13C] ribonucleoside phosphoramidites has been achieved.
摘要
本文介绍了从D-[1-¹³C]核糖固相合成特定¹'-¹³C标记的寡核糖核苷酸所用单体构建块的合成方法。该方法已用于选择性形成2'-O-硅烷化核糖核苷。在5'-O-二甲氧基三苯甲基化后，实现了D-[1'-¹³C]核糖核苷亚磷酰胺的合成。
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