Gerlini G, Hefti H P, Kleinhans M, Nickoloff B J, Burg G, Nestle F O
Department of Dermatology, University of Zürich Medical School, Zürich, Switzerland.
J Invest Dermatol. 2001 Sep;117(3):576-82. doi: 10.1046/j.0022-202x.2001.01458.x.
CD1 proteins are a family of cell surface molecules that present lipid antigens to T cells. We investigated skin dendritic cells and monocyte-derived dendritic cells for expression of CD1 molecules using a panel of 10 different monoclonal antibodies focusing on the recently described CD1d molecule. By immunohistochemical analysis, CD1d expression in normal human skin was restricted to dendritic appearing cells in the papillary dermis mainly located in a perivascular localization. Langerhans cells did not show detectable CD1d expression in situ. Epidermal/dermal cell suspensions analyzed by flow cytometry demonstrated distinct subpopulations of HLA-DR positive dermal dendritic cells expressing CD1a, CD1b, and CD1c. CD1d was expressed on HLA-DRbright dermal antigen-presenting cells in dermal suspensions (16% +/- 3.6%), as well as on highly enriched dermal dendritic cells migrating out of skin explants (60.5% +/- 8.0%). Migrated mature dermal dendritic cells coexpressed CD83 and CD1d. Western blot analysis on microdissected skin sections revealed the presence of a 50-55 kDa CD1d molecule in dermis, suggesting that CD1d is highly glycosylated in skin. Both immature and mature monocyte-derived dendritic cells cultured in autologous plasma expressed CD1d molecules. In contrast, culture in fetal bovine serum downregulated CD1d expression. In conclusion, antigen-presenting cells in skin express different sets of CD1 molecules including CD1d and might play a role in lipid antigen presentation in various skin diseases. Differential expression of CD1 molecules depending on culture conditions might have an impact on clinical applications of dendritic cells for immunotherapy.
CD1蛋白是一类细胞表面分子，可将脂质抗原呈递给T细胞。我们使用一组针对最近描述的CD1d分子的10种不同单克隆抗体，研究了皮肤树突状细胞和单核细胞衍生的树突状细胞中CD1分子的表达情况。通过免疫组织化学分析，正常人皮肤中的CD1d表达仅限于乳头真皮中呈树突状的细胞，主要位于血管周围。原位观察时，朗格汉斯细胞未显示出可检测到的CD1d表达。通过流式细胞术分析的表皮/真皮细胞悬液显示，表达CD1a、CD1b和CD1c的HLA-DR阳性真皮树突状细胞存在不同亚群。CD1d在真皮悬液中的HLA-DRbright真皮抗原呈递细胞上表达（16%±3.6%），也在从皮肤外植体迁移出的高度富集的真皮树突状细胞上表达（60.5%±8.0%）。迁移的成熟真皮树突状细胞共表达CD83和CD1d。对显微切割的皮肤切片进行的蛋白质印迹分析显示真皮中存在50 - 55 kDa的CD1d分子，这表明CD1d在皮肤中高度糖基化。在自体血浆中培养的未成熟和成熟单核细胞衍生的树突状细胞均表达CD1d分子。相比之下，在胎牛血清中培养会下调CD1d表达。总之，皮肤中的抗原呈递细胞表达包括CD1d在内的不同组CD1分子，可能在各种皮肤疾病的脂质抗原呈递中发挥作用。CD1分子根据培养条件的差异表达可能会对树突状细胞用于免疫治疗的临床应用产生影响。
