Lenander C, Habermann J K, Ost A, Nilsson B, Schimmelpenning H, Tryggvason K, Auer G
Department of Surgery, Ersta Hospital, Stockholm, Sweden.
Anal Cell Pathol. 2001;22(4):201-9. doi: 10.1155/2001/137404.
Expression of the gamma 2 chain at the invasive front of different tumors has indicated an important role for laminin-5 in cell migration during tumor invasion and tissue remodeling. As there is considerable need for reliable invasion and prognostic markers we evaluated the correlation of laminin-5 gamma 2 chain expression with clinicopathologic parameters and patient survival in 93 primary colon carcinomas. Epithelial cells of normal mucosa were consistently negative for staining. In contrast, positive cytoplasmic staining was observed in 89 tumors (96%). Twenty-four (26%) cases were scored as sparse, 34 (37%) as moderate, and 31 (33%) as frequent gamma 2 chain expression. There was a significant association of laminin-5 gamma 2 chain expression and local invasiveness of colon carcinomas according to Dukes stage (A-C) (p=0.001) and tumor budding (p<0.001). A statistical significance could also be noted in decreasing tumor differentiation (p<0.001) and correlation to tumor size (p=0.032). No correlation was observed to tumor site. Univariate analysis identified laminin-5 (p=0.010), tumor differentiation (p=0.006) and Dukes grade (p<0.001) as significant variables in predicting prognosis. However, by multivariate analyses, this study could not demonstrate that laminin-5 gamma 2 chain expression is an independent predictive factor for survival. The results indicate that laminin-5 gamma 2 chain expression is up-regulated during the progression of human colon cancer and that it plays a role in the aggressiveness of these tumors. Demonstration of laminin-5 gamma 2 chain positivity also facilitates detection of individual cells or minor cell clusters invading the surrounding stroma. Figures on http://www.esacp.org/acp/2001/22-4/lenander.htm.
γ2链在不同肿瘤侵袭前沿的表达表明层粘连蛋白-5在肿瘤侵袭和组织重塑过程中的细胞迁移中起重要作用。由于对可靠的侵袭和预后标志物有相当大的需求，我们评估了93例原发性结肠癌中层粘连蛋白-5γ2链表达与临床病理参数及患者生存率的相关性。正常黏膜上皮细胞染色始终为阴性。相比之下，89例肿瘤（96%）观察到细胞质阳性染色。24例（26%）病例γ2链表达评分为稀疏，34例（37%）为中等，31例（33%）为频繁表达。根据Dukes分期（A - C）（p = 0.001）和肿瘤芽生（p < 0.001），层粘连蛋白-5γ2链表达与结肠癌的局部侵袭性有显著相关性。在肿瘤分化降低（p < 0.001）和与肿瘤大小的相关性（p = 0.032）方面也可观察到统计学意义。未观察到与肿瘤部位的相关性。单因素分析确定层粘连蛋白-5（p = 0.010）、肿瘤分化（p = 0.006）和Dukes分级（p < 0.001）是预测预后的重要变量。然而，通过多因素分析，本研究未能证明层粘连蛋白-5γ2链表达是生存的独立预测因素。结果表明层粘连蛋白-5γ2链表达在人类结肠癌进展过程中上调，并且它在这些肿瘤的侵袭性中起作用。层粘连蛋白-5γ2链阳性的显示也有助于检测侵入周围基质的单个细胞或小细胞簇。图见http://www.esacp.org/acp/2001/22-4/lenander.htm。
