Hughes P, Holt S, Rowell N R
Br J Dermatol. 1975 Apr;92(4):401-6. doi: 10.1111/j.1365-2133.1975.tb03100.x.
The leukocyte migration test was used to examine patients with systemic lupus erythematosus (SLE) for evidence of cell-mediated immunity to human liver microsomes and mitochondria. Testing of both these antigens with leukocytes from patients was carried out simultaneously in culture medium enriched by either 20% fetal bovine serum (the standard test) or substituted 20% autologous serum. Normal controls showed little or no reactivity in the standard test whereas the SLE group revealed a high incidence of significant leukocyte migration inhibition to both antigens which, however, was considerably reduced by the substitution of autologous serum. The results suggest that the sera of patients with SLE contain factor(s) capable of blocking the in vitro expression of cell-mediated immunity to human tissue antigens. These factor(s), if active in vivo, might have a role in controlling some of the auto-immune manfestations of the disease.
采用白细胞迁移试验检测系统性红斑狼疮（SLE）患者对人肝微粒体和线粒体的细胞介导免疫证据。在添加20%胎牛血清（标准试验）或替代为20%自体血清的培养基中，同时用患者的白细胞对这两种抗原进行检测。正常对照在标准试验中几乎没有或没有反应，而SLE组对两种抗原均显示出显著的白细胞迁移抑制的高发生率，然而，用自体血清替代后，这种抑制作用明显降低。结果表明，SLE患者的血清中含有能够阻断对人组织抗原的细胞介导免疫体外表达的因子。这些因子若在体内具有活性，可能在控制该疾病的某些自身免疫表现中起作用。
