大肠杆菌核糖核酸酶P的催化成分M1 RNA的3'加工过程中，rne依赖性位点序列的作用。

Role of the sequence of the rne-dependent site in 3' processing of M1 RNA, the catalytic component of Escherichia coli RNase P.

作者信息

Sim S, Kim S, Lee Y

机构信息

Department of Chemistry and Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology, Taejon 305-701, South Korea.

出版信息

FEBS Lett. 2001 Sep 14;505(2):291-5. doi: 10.1016/s0014-5793(01)02822-8.

DOI:10.1016/s0014-5793(01)02822-8

PMID:11566192

Abstract

The 3' processing of M1 RNA, the catalytic component of Escherichia coli RNase P, occurs by two pathways involving multiple steps. The precursor of M1 RNA has an rne-dependent site downstream of the processing site, whose sequence variation affects the processing efficiency. In this study, we showed that the sequence itself of the rne-dependent site possessed the ability to determine the processing pathways and that it also affected the cleavage specificity with the generation of the processing products at one nucleotide upstream or downstream of the normal cleavage sites.

摘要

大肠杆菌核糖核酸酶P的催化成分M1 RNA的3' 加工通过涉及多个步骤的两条途径进行。M1 RNA的前体在加工位点下游有一个依赖rne的位点，其序列变异会影响加工效率。在本研究中，我们表明，依赖rne的位点的序列本身具有决定加工途径的能力，并且它还通过在正常切割位点上游或下游一个核苷酸处产生加工产物来影响切割特异性。