小模型底物被修饰的核糖核酸酶P切割的要求。
Requirements for cleavage by a modified RNase P of a small model substrate.
作者信息
Liu F, Altman S
机构信息
Department of Biology, Yale University, New Haven, CT 06520, USA.
出版信息
Nucleic Acids Res. 1996 Jul 15;24(14):2690-6. doi: 10.1093/nar/24.14.2690.
Abstract
M1 RNA, the catalytic RNA subunit of RNase P from Escherichia coli, has been covalently linked at its 3' terminus to oligonucleotides (guide sequences) that guide the enzyme to target RNAs through hybridization with the target sequences. These constructs (M1GS RNAs) have been used to determine some minimal features of model substrates. As few as 3 bp on the 3' side of the site of cleavage in a substrate complex and 1 nt on the 5' side are required for cleavage to occur. The cytosines in the 3' terminal CCA sequence of the model substrates are important for cleavage efficiency but not cleavage site selection. A purine (base-paired or not) at the 3' side of the cleavage site is important both for cleavage site selection and efficiency. M1GS RNAs provide both a simple system for characterization of the reaction governed by M1 RNA and a tool for gene therapy.
摘要
M1 RNA是来自大肠杆菌的核糖核酸酶P的催化性RNA亚基，已在其3'末端与寡核苷酸（引导序列）共价连接，这些寡核苷酸通过与靶序列杂交将该酶引导至靶RNA。这些构建体（M1GS RNA）已用于确定模型底物的一些最小特征。底物复合物中切割位点3'侧少至3个碱基对和5'侧少至1个核苷酸是切割发生所必需的。模型底物3'末端CCA序列中的胞嘧啶对切割效率很重要，但对切割位点选择不重要。切割位点3'侧的嘌呤（无论是否碱基配对）对切割位点选择和效率都很重要。M1GS RNA既为表征由M1 RNA控制的反应提供了一个简单系统，也为基因治疗提供了一种工具。
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本文引用的文献
1
Probing of tertiary interactions in RNA: 2'-hydroxyl-base contacts between the RNase P RNA and pre-tRNA.RNA中三级相互作用的探究：核糖核酸酶P RNA与前体tRNA之间的2'-羟基-碱基接触
Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12510-4. doi: 10.1073/pnas.92.26.12510.
2
Cleavage by RNase P of gene N mRNA reduces bacteriophage lambda burst size.核糖核酸酶P对基因N信使核糖核酸的切割会降低噬菌体λ的爆发量。
Nucleic Acids Res. 1996 Mar 1;24(5):835-42. doi: 10.1093/nar/24.5.835.
3
Multiple magnesium ions in the ribonuclease P reaction mechanism.核糖核酸酶P反应机制中的多个镁离子
Biochemistry. 1993 May 25;32(20):5273-81. doi: 10.1021/bi00071a001.
4
Artificial self-cleaving molecules consisting of a tRNA precursor and the catalytic RNA of RNase P.由tRNA前体和核糖核酸酶P的催化RNA组成的人工自我切割分子。
Nucleic Acids Res. 1993 Oct 11;21(20):4685-9. doi: 10.1093/nar/21.20.4685.
5
Rational design of self-cleaving pre-tRNA-ribonuclease P RNA conjugates.自我切割前体tRNA核糖核酸酶P RNA缀合物的合理设计。
Biochemistry. 1994 Sep 6;33(35):10800-8. doi: 10.1021/bi00201a030.
6
Differential evolution of substrates for an RNA enzyme in the presence and absence of its protein cofactor.在存在和不存在其蛋白质辅因子的情况下，RNA酶底物的差异进化。
Cell. 1994 Jul 1;77(7):1093-100. doi: 10.1016/0092-8674(94)90448-0.
7
Recent studies of ribonuclease P.核糖核酸酶P的近期研究。
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8
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Science. 1995 Feb 3;267(5198):675-9. doi: 10.1126/science.7839142.
9
Pathway of activation by magnesium ions of substrates for the catalytic subunit of RNase P from Escherichia coli.大肠杆菌核糖核酸酶P催化亚基底物被镁离子激活的途径。
J Mol Biol. 1993 Apr 5;230(3):750-6. doi: 10.1006/jmbi.1993.1197.
10
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