聚丙氨酸肽的膜插入与取向：一项利用（15）N 固态核磁共振光谱学的研究
Membrane insertion and orientation of polyalanine peptides: a (15)N solid-state NMR spectroscopy investigation.
作者信息
Bechinger B
机构信息
Max Planck Institute for Biochemistry, 82152 Martinsried, Germany.
出版信息
Biophys J. 2001 Oct;81(4):2251-6. doi: 10.1016/S0006-3495(01)75872-9.
Abstract
Polyalanine-based peptides were prepared by solid-phase peptide synthesis, labeled with (15)N at selected sites, reconstituted into oriented phosphatidylcholine bilayers, and investigated by proton-decoupled (15)N solid-state NMR spectroscopy. The anisotropic (15)N chemical shift is a direct indicator of helix alignment with respect to the membrane normal. The in-plane to transmembrane equilibrium is the focus of this study. Time- and solvent-dependent transmembrane alignments of K(3)A(18)K(3) have been obtained, and these are stabilized when a few alanine residues are replaced with leucine. The results are discussed in the context of a model where polyalanines adopt a variety of configurations, which are interconnected by multiple equilibria. The data indicate hydrophobicity values of alanine close to zero when studied in the context of helical polypeptides (> or =24 residues) and phospholipid bilayers.
摘要
基于聚丙氨酸的肽通过固相肽合成制备，在选定位置用（15）N标记，重构成定向磷脂酰胆碱双层膜，并通过质子去耦（15）N固态核磁共振光谱进行研究。各向异性（15）N化学位移是螺旋相对于膜法线排列的直接指标。面内到跨膜的平衡是本研究的重点。已获得K（3）A（18）K（3）随时间和溶剂变化的跨膜排列，当几个丙氨酸残基被亮氨酸取代时，这些排列会稳定下来。在一个模型的背景下讨论了结果，该模型中聚丙氨酸采用多种构型，这些构型通过多个平衡相互连接。数据表明，在螺旋多肽（≥24个残基）和磷脂双层膜的背景下研究时，丙氨酸的疏水性值接近零。
相似文献
1
Membrane insertion and orientation of polyalanine peptides: a (15)N solid-state NMR spectroscopy investigation.聚丙氨酸肽的膜插入与取向：一项利用（15）N 固态核磁共振光谱学的研究
Biophys J. 2001 Oct;81(4):2251-6. doi: 10.1016/S0006-3495(01)75872-9.
2
Alignment of lysine-anchored membrane peptides under conditions of hydrophobic mismatch: a CD, 15N and 31P solid-state NMR spectroscopy investigation.疏水错配条件下赖氨酸锚定膜肽的排列：圆二色光谱、15N和31P固态核磁共振光谱研究
Biochemistry. 2000 Oct 31;39(43):13106-14. doi: 10.1021/bi000770n.
3
Structure and alignment of the membrane-associated peptaibols ampullosporin A and alamethicin by oriented 15N and 31P solid-state NMR spectroscopy.通过定向 15N 和 31P 固态 NMR 光谱学研究膜相关缩肽类抗生素ampullosporin A 和 alamethicin 的结构和排列。
Biophys J. 2009 Jan;96(1):86-100. doi: 10.1529/biophysj.108.136242.
4
Investigations of polypeptide rotational diffusion in aligned membranes by 2H and 15N solid-state NMR spectroscopy.通过2H和15N固态核磁共振光谱法研究多肽在有序排列膜中的旋转扩散。
J Am Chem Soc. 2004 Dec 22;126(50):16676-83. doi: 10.1021/ja0468675.
5
Topological equilibria of ion channel peptides in oriented lipid bilayers revealed by 15N solid-state NMR spectroscopy.通过15N固态核磁共振光谱揭示的定向脂质双层中离子通道肽的拓扑平衡。
Biochemistry. 2005 Sep 13;44(36):12120-7. doi: 10.1021/bi050866n.
6
The topology of lysine-containing amphipathic peptides in bilayers by circular dichroism, solid-state NMR, and molecular modeling.通过圆二色性、固态核磁共振和分子模拟研究含赖氨酸两亲性肽在双分子层中的拓扑结构。
Biophys J. 2000 Nov;79(5):2644-56. doi: 10.1016/S0006-3495(00)76503-9.
7
Tilt and rotational pitch angle of membrane-inserted polypeptides from combined 15N and 2H solid-state NMR spectroscopy.结合15N和2H固态核磁共振光谱法测定膜插入多肽的倾斜角和旋转俯仰角
Biochemistry. 2004 Aug 17;43(32):10502-12. doi: 10.1021/bi049409h.
8
Solid state NMR studies of oligourea foldamers: interaction of 15N-labelled amphiphilic helices with oriented lipid membranes.寡聚脲折叠物的固态 NMR 研究：15N 标记的两亲性螺旋与定向脂质膜的相互作用。
Org Biomol Chem. 2012 Feb 21;10(7):1440-7. doi: 10.1039/c1ob06278f. Epub 2012 Jan 4.
9
Structure and orientation of antibiotic peptide alamethicin in phospholipid bilayers as revealed by chemical shift oscillation analysis of solid state nuclear magnetic resonance and molecular dynamics simulation.通过固态核磁共振的化学位移振荡分析和分子动力学模拟揭示的抗生素肽阿拉米辛在磷脂双层中的结构和取向
Biochim Biophys Acta. 2015 Nov;1848(11 Pt A):2789-98. doi: 10.1016/j.bbamem.2015.07.019. Epub 2015 Aug 3.
10
Interactions of two transmembrane peptides in supported lipid bilayers studied by a (31)P and (15)N MAOSS NMR strategy.通过磷-31和氮-15魔角旋转固体核磁共振策略研究支撑脂质双分子层中两种跨膜肽的相互作用。
Biochimie. 2009 Jun;91(6):774-8. doi: 10.1016/j.biochi.2009.01.009.
引用本文的文献
1
Development of Nontoxic Peptides for Lipopolysaccharide Neutralization and Sepsis Treatment.用于中和脂多糖及治疗脓毒症的无毒肽的研发
ACS Pharmacol Transl Sci. 2024 May 21;7(6):1795-1806. doi: 10.1021/acsptsci.4c00033. eCollection 2024 Jun 14.
2
Solid-State NMR Investigations of the MHC II Transmembrane Domains: Topological Equilibria and Lipid Interactions.固态 NMR 研究 MHC II 跨膜结构域：拓扑平衡和脂质相互作用。
J Membr Biol. 2019 Oct;252(4-5):371-384. doi: 10.1007/s00232-019-00071-8. Epub 2019 Jun 11.
3
The next generation of antimicrobial peptides (AMPs) as molecular therapeutic tools for the treatment of diseases with social and economic impacts.下一代抗菌肽作为治疗具有社会和经济影响疾病的分子治疗工具。
Drug Discov Today. 2017 Feb;22(2):234-248. doi: 10.1016/j.drudis.2016.10.017. Epub 2016 Nov 23.
4
Sensitivity and resolution enhancement of oriented solid-state NMR: application to membrane proteins.提高各向异性固态 NMR 的灵敏度和分辨率：在膜蛋白中的应用。
Prog Nucl Magn Reson Spectrosc. 2013 Nov;75:50-68. doi: 10.1016/j.pnmrs.2013.07.004. Epub 2013 Aug 12.
5
Membrane partitioning of the pore-forming domain of colicin A. Role of the hydrophobic helical hairpin.细菌素 A 孔形成域的膜分区。疏水性发夹螺旋的作用。
Biophys J. 2013 Sep 17;105(6):1432-43. doi: 10.1016/j.bpj.2013.08.012.
6
Tether-supported biomembranes with α-helical peptide-based anchoring constructs.带支撑的生物膜，具有基于α-螺旋肽的锚固结构。
Langmuir. 2013 Jan 8;29(1):299-307. doi: 10.1021/la303628n. Epub 2012 Dec 21.
7
Contribution of topology determinants of a viral movement protein to its membrane association, intracellular traffic, and viral cell-to-cell movement.病毒运动蛋白拓扑结构决定因素对其膜结合、细胞内运输和病毒细胞间运动的贡献。
J Virol. 2011 Aug;85(15):7797-809. doi: 10.1128/JVI.02465-10. Epub 2011 May 18.
8
Peptide model helices in lipid membranes: insertion, positioning, and lipid response on aggregation studied by X-ray scattering.X 射线散射研究肽模型螺旋在脂质膜中的插入、定位和脂质响应聚集
Eur Biophys J. 2011 Apr;40(4):417-36. doi: 10.1007/s00249-010-0645-4. Epub 2010 Dec 23.
9
Effect of lipid composition on the topography of membrane-associated hydrophobic helices: stabilization of transmembrane topography by anionic lipids.脂质组成对膜相关疏水螺旋结构的影响：阴离子脂质对跨膜结构的稳定作用。
J Mol Biol. 2008 Jun 13;379(4):704-18. doi: 10.1016/j.jmb.2008.04.026. Epub 2008 Apr 16.
10
The control of transmembrane helix transverse position in membranes by hydrophilic residues.亲水性残基对膜中跨膜螺旋横向位置的控制。
J Mol Biol. 2007 Dec 14;374(5):1251-69. doi: 10.1016/j.jmb.2007.10.032. Epub 2007 Oct 17.
本文引用的文献
1
The topology of lysine-containing amphipathic peptides in bilayers by circular dichroism, solid-state NMR, and molecular modeling.通过圆二色性、固态核磁共振和分子模拟研究含赖氨酸两亲性肽在双分子层中的拓扑结构。
Biophys J. 2000 Nov;79(5):2644-56. doi: 10.1016/S0006-3495(00)76503-9.
2
Alignment of lysine-anchored membrane peptides under conditions of hydrophobic mismatch: a CD, 15N and 31P solid-state NMR spectroscopy investigation.疏水错配条件下赖氨酸锚定膜肽的排列：圆二色光谱、15N和31P固态核磁共振光谱研究
Biochemistry. 2000 Oct 31;39(43):13106-14. doi: 10.1021/bi000770n.
3
Solid-state NMR spectroscopy applied to membrane proteins.应用于膜蛋白的固态核磁共振光谱学。
Curr Opin Struct Biol. 2000 Oct;10(5):593-600. doi: 10.1016/s0959-440x(00)00136-6.
4
Membrane interactions and alignment of structures within the HIV-1 Vpu cytoplasmic domain: effect of phosphorylation of serines 52 and 56.
FEBS Lett. 2000 Oct 6;482(3):220-4. doi: 10.1016/s0014-5793(00)02060-3.
5
Crystal structure of rhodopsin: A G protein-coupled receptor.视紫红质的晶体结构：一种G蛋白偶联受体。
Science. 2000 Aug 4;289(5480):739-45. doi: 10.1126/science.289.5480.739.
6
Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 A resolution.肌浆网钙泵在2.6埃分辨率下的晶体结构。
Nature. 2000 Jun 8;405(6787):647-55. doi: 10.1038/35015017.
7
The structure, dynamics and orientation of antimicrobial peptides in membranes by multidimensional solid-state NMR spectroscopy.利用多维固态核磁共振光谱研究抗菌肽在膜中的结构、动力学和取向
Biochim Biophys Acta. 1999 Dec 15;1462(1-2):157-83. doi: 10.1016/s0005-2736(99)00205-9.
8
Peptide structural analysis by solid-state NMR spectroscopy.通过固态核磁共振光谱法进行肽结构分析。
Biopolymers. 1999;51(3):174-90. doi: 10.1002/(SICI)1097-0282(1999)51:3<174::AID-BIP2>3.0.CO;2-7.
9
Design and characterization of anchoring amphiphilic peptides and their interactions with lipid vesicles.锚定两亲性肽的设计、表征及其与脂质囊泡的相互作用。
Biopolymers. 1999 Nov;50(6):647-55. doi: 10.1002/(SICI)1097-0282(199911)50:6<647::AID-BIP9>3.0.CO;2-T.
10
Beta-barrel proteins from bacterial outer membranes: structure, function and refolding.细菌外膜中的β-桶状蛋白：结构、功能与重折叠
Curr Opin Struct Biol. 1999 Aug;9(4):455-61. doi: 10.1016/S0959-440X(99)80064-5.
Zotero 插件